子痫前期胎盘中AQPs家族分子表达与细胞侵袭、凋亡的相关性研究

The correlation of AQPs family molecule expression with cell invasion and apoptosis in preeclampsia placenta

目的:研究子痫前期(PE)胎盘中水通道蛋白(AQPs)家族分子表达与细胞侵袭、凋亡的相关性。方法:选择2014年7月~2017年5月期间在绵阳市中心医院产科诊断为子痫前期的产妇,并根据病情严重程度分为轻度PE组和重度PE组,选择同期在绵阳市中心医院产检及分娩的健康产妇作为对照组。收集胎盘并测定AQPs家族分子、细胞侵袭分子、细胞凋亡分子的mRNA表达量。结果:重度PE组和轻度PE组胎盘组织中AQP1、AQP3、AQP8、AQP9、TXNIP、NLRP3、Fas、HSP70、Caspase-3的mRNA表达量均显著高于对照组(P<0.05),LAMA4、GRP30、OPN、EMMPRIN、MMP2、MMP9的mRNA表达量均显著低于对照组(P<0.05);重度PE组胎盘组织中AQP1、AQP3、AQP8、AQP9、TXNIP、NLRP3、Fas、HSP70、Caspase-3的mRNA表达量均显著高于轻度PE组(P<0.05),LAMA4、GRP30、OPN、EMMPRIN、MMP2、MMP9的mRNA表达量均显著低于轻度PE组(P<0.05);PE胎盘中AQP1、AQP3、AQP8、AQP9的mRNA表达量与LAMA4、GRP30、OPN、EMMPRIN、MMP2、MMP9的mRNA表达量呈负相关,与TXNIP、NLRP3、Fas、HSP70、Caspase-3的mRNA表达量呈正相关。结论:子痫前期胎盘中高表达的AQP1、AQP3、AQP8、AQP9能够抑制滋养细胞的侵袭、促进滋养细胞的凋亡。

Objective:To study the correlation of aquaporins(AQPs)family molecule expression with cell invasion and apoptosis in preeclampsia placenta.Methods:The puerperae who were diagnosed with preeclampsia in Obstetrics Department of Mianyang Central Hospital between July2014and May2017were selected and divided into mild PE group and severe PE group according to the disease severity,and the healthy puerperae who received antenatal care and gave birth in Mianyang Central Hospital over the same period were selected as control group.The placenta was collected to determine the mRNA expression of AQPs family molecules,cell invasion molecules and apoptosis molecules.Results:AQP1,AQP3,AQP8,AQP9,TXNIP,NLRP3,Fas,HSP70and Caspase-3mRNA expression in placenta tissue of severe PE group and mild PE group were greatly higher than those of control group whereas LAMA4,GRP30,OPN,EMMPRIN,MMP2and MMP9mRNA expression were greatly lower than those of control group;AQP1,AQP3,AQP8,AQP9,TXNIP,NLRP3,Fas,HSP70and Caspase-3mRNA expression in placenta tissue of severe PE group were greatly higher than those of mild PE group whereas LAMA4,GRP30,OPN,EMMPRIN,MMP2and MMP9mRNA expression were greatly lower than those of mild PE group;AQP1,AQP3,AQP8and AQP9mRNA expression in placenta of PE were negatively correlated with LAMA4,GRP30,OPN,EMMPRIN,MMP2and MMP9mRNA expression,and positively correlated with TXNIP,NLRP3,Fas,HSP70and Caspase-3mRNA expression.Conclusion:Highly expressed AQP1,AQP3,AQP8and AQP9can inhibit the invasion of trophoblast cells and promote the apoptosis of trophoblast cells in the placenta of preeclampsia.