小肝癌超声造影参数与癌基因、血管新生基因表达的相关性

The correlation of small hepatocellular carcinoma ultrasonography parameters with the expression of oncogenes and angiogenesis genes

目的:探讨小肝癌超声造影参数与癌基因、血管新生基因表达的相关性。方法:选择2015年7月~2017年5月在本院确诊的小肝癌患者61例作为小肝癌组,肝内胆管结石患者48例作为肝内胆管结石组。记录两组患者的超声造影参数,检测手术病灶组织中癌基因、血管新生基因的表达量。采用Pearson检验评估小肝癌患者超声造影参数水平与癌基因、血管新生基因表达量的相关关系。结果:小肝癌组的IMAX水平高于肝内胆管结石组,TTP、mTT的水平低于肝内胆管结石组;病灶组织中癌基因C-myc、N-ras、PIK3CA、RMP、Bmil、pim-3mRNA的表达量均高于肝内胆管结石组;病灶组织中血管新生基因VEGF、Ang-1、Tie-2、MACC1mRNA的表达量均高于肝内胆管结石组,ARH1mRNA的表达量低于肝内胆管结石组。Pearson检验发现,小肝癌患者超声造影参数IMAX、TTP、mTT水平与病灶癌基因、血管新生基因表达量直接相关。结论:小肝癌患者超声造影参数与良性疾病患者差异巨大,且具体参数水平与癌细胞恶性程度直接相关。

Objective:To investigate the correlation of small hepatocellular carcinoma ultrasonography parameters with the expression of oncogenes and angiogenesis genes.Methods:A total of61patients with small hepatocellular carcinoma who were diagnosed in this hospital between July2015and May2017were selected as small hepatocellular carcinoma group,and48patients with hepatolithiasis were selected as hepatolithiasis group.The ultrasonography parameters of the two groups were recorded and the expression levels of oncogenes and angiogenesis genes in the surgical lesion tissues were detected.Pearson test was used to evaluate the correlation of ultrasonography parameters with the expression of oncogenes and angiogenesis genes.Results:IMAX level in small hepatocellular carcinoma group was higher than that in hepatolithiasis group while TTP and mTT levels were lower than those in hepatolithiasis group;oncogenes C-myc,N-ras,PIK3CA,RMP,Bmil and pim-3mRNA expression in lesion tissues were higher than those of hepatolithiasis group;angiogenesis genes VEGF,Ang-1,Tie-2and MACC1mRNA expression in lesion tissues were higher than those of hepatolithiasis group while ARH1mRNA expression was lower than that of hepatolithiasis group.Pearson test showed that the ultrasonography parameters IMAX,TTP and mTT levels in patients with small hepatocellular carcinoma were directly correlated to the expression of oncogenes and angiogenesis genes in lesions.Conclusion:The ultrasonography parameters of patients with small hepatocellular carcinoma are significantly different from those of patients with benign diseases,and the specific parameter levels are directly correlated with the malignancy of cancer cells.