超声造影参数对乳腺肿瘤性质的评估价值及其与肿瘤恶性生物学行为的相关关系

The assessment value of contrast-enhanced ultrasound parameters for the nature of breast tumor and their relationship with the malignant biological behavior of tumor

目的:探讨超声造影参数对乳腺肿瘤性质的评估价值及其与肿瘤恶性生物学行为的相关关系。方法:2015年1月~2017年1月间在本院进行手术治疗的乳腺肿瘤患者176例,根据病理检查结果分为乳腺腺瘤组110例、乳腺癌组66例。对比两组患者的乳腺超声造影参数水平,肿瘤组织中增殖、侵袭、自噬基因表达量的差异。采用Pearson检验评估乳腺癌患者超声造影参数与病灶组织恶性分子表达量的相关关系。结果:乳腺癌组的PI、AUC水平显著高于乳腺腺瘤组;肿瘤组织中增殖基因CXCL1、Notch1mRNA的表达量高于乳腺腺瘤组,HPK1 mRNA的表达量低于乳腺腺瘤组;肿瘤组织中侵袭基因Gab2、NUAK1、FOXF1mRNA的表达量高于乳腺腺瘤组,NDRG1 mRNA的表达量低于乳腺腺瘤组;肿瘤组织中自噬基因ATG2B、ATG4D、ATG9B mRNA的表达量低于乳腺腺瘤组。经Pearson检验发现,乳腺癌患者超声造影参数PI、AUC水平与病灶组织中增殖、侵袭、自噬等分子表达量直接相关。结论:乳腺癌患者超声造影参数水平异于良性肿瘤者,且具体参数水平与肿瘤恶性程度直接相关,可作为早期筛查疾病、评估恶性程度的可靠手段。

Objective:To discuss the value of contrast-enhanced ultrasound parameters for assessing the nature of breast tumor and their relationship with the malignant biological behavior of tumor.Methods:Total of176patients with breast tumor who received surgical treatment in the hospital between January2015and January2017were divided into breast adenoma group(n=110)and breast cancer group(n=66)according to pathological results.The differences in breast contrast-enhanced ultrasound parameter levels as well as the expression of proliferation,invasion and autophagy genes in tumor tissues were compared between the two groups of patients.Pearson test was used to evaluate the correlation between contrast-enhanced ultrasound parameters and malignant molecule expression in lesion tissue of patients with breast cancer.Results:PI and AUC levels in breast cancer group were greatly higher than those in breast adenoma group;proliferation genes CXCL1and Notch1mRNA expression in tumor tissue were higher than those of breast adenoma group whereas HPK1mRNA expression was lower than that of breast adenoma group;invasion genes Gab2,NUAK1and FOXF1mRNA expression in tumor tissue were higher than those of breast adenoma group whereas NDRG1mRNA expression was lower than that of breast adenoma group;autophagy genes ATG2B,ATG4D and ATG9B mRNA expression in tumor tissue were lower than those of breast adenoma group.Pearson test showed that the contrast-enhanced ultrasound parameters PI and AUC levels in patients wit breast cancer were directly correlated with the expression of proliferation,invasion and autophagy molecules in the lesion tissue.Conclusions:The contrast-enhanced ultrasound parameter levels in patients with breast cancer are different from those in patients with benign tumors,and the specific parameter levels are directly correlated with the tumor malignancy and can be used as the reliable means for early disease screening and malignancy assessment.