Mn_3[Co(CN)_6]_2@SiO_2对鼠C6脑胶质瘤模型3T MR成像及生长规律的研究

Application of Mn_3[Co(CN)_6]_2@SiO_2 on magnetic resonance imaging and growth regulation of rat C6 gliomas

目的利用Mn_3[Co(CN)_6]_2@SiO_2对大鼠C6脑胶质瘤模型进行3T MR成像,研究肿瘤的生长规律。方法收集对数生长期鼠C6胶质瘤细胞,建立鼠C6脑胶质瘤模型,于细胞接种后第7 d行MR扫描,然后鼠尾静脉注射0.5 mL Mn_3[Co(CN)_6]_2@SiO_2,于注射后5 min、24 h再行MR扫描,扫描结束后取出肿瘤组织行HE染色。随机抽取脑内成功接种C6胶质瘤细胞的荷瘤鼠10只,接种后第7、9、11、13、15、17、19天行MR扫描,测量肿瘤体积,绘制出肿瘤生长曲线。结果成功建立了鼠C6脑胶质瘤模型,并且鼠尾静脉注射Mn_3[Co(CN)_6]_2@SiO_2后5 min、24 h肿瘤均强化,24 h肿瘤强化较前明显;MRI检测出胶质瘤大小与时间呈正相关关系(rs=0.9944,P=0.000)。结论应用Mn_3[Co(CN)_6]_2@SiO_2对鼠C6脑胶质瘤活体示踪MR成像,可以反映肿瘤的生物学特性,适用于肿瘤生长规律的观测。

ObjectiveTo evaluate the imaging features of glioma and growth regulation of this nanocarrier with the aid of HE staining via using the rat C6brain glioma model with Mn3[Co(CN)6]2@SiO2enhanced imaging at3T MRI.MethodsThe model of rat C6brain glioma was built with glioma cells in the logarithmic growth stage.Seven days after cellimplantation,MR imaging was performed at0,5m,24h after0.5mL Mn3[Co(CN)6]2@SiO2was injected through the tail vein.Rat brain tissues were obtained and examined by HE staining.MR images of10rats with C6tumor glioma were performed on7,9,11,13,15,17,19days after glioma implantation.Then the images were transmitted to ADW4.4workstation.The longest diameter and widest diameter of the tumor were measured and the tumor volume was calculated,and the growth regulations in animal models were evaluated.ResultsThe models of rat C6brain glioma models were successfully established,and the MR images with Mn3[Co(CN)6]2@SiO2were obtained.A positive correlation was detected between tumor size and time of glioma by MRI.ConclusionWith the help of Mn3[Co(CN)6]2@SiO2,MRI is able to trace the growth character of rat C6glioma and evaluate the growth regulation in animal models.