笃斯越橘花青苷合成酶基因(ANR)的克隆与序列分析

Cloning and sequence analysis is of anthocyanidin reductase gene(ANR) fromVaccinium uliginosum

以笃斯越橘的盛花期花朵为试材,克隆了笃斯越橘花青苷合成途径ANR基因并对其进行序列分析,结果表明:笃斯越橘ANR基因的cDNA序列全长为1 226bp,其中包含1 035bp ORF,编码344个氨基酸,相对分子质量为38kd,等电点值为5.34。将其氨基酸序列与其他植物的ANR基因比对后发现,笃斯越橘ANR基因分别与桃、苹果、葡萄、野草莓的ANR基因序列的一致性达61%、62%、62%和59%,表明克隆到了正确的笃斯越橘ANR基因。该序列与其他植物的ANR基因序列构建的系统发育树表明,所克隆的笃斯越橘ANR基因分别与桃、苹果、野草莓等亲缘关系较近。

With Vaccinium uliginosum in full bloom stage as materials,anthocyanin biosynthesis ANR gene was cloned and analyzed.The results showed that the length of DNA sequence of the ANR gene was1226bp,which contains1035bp open reading frame encoding a protein comprising of344amino acids with a predicted molecular weight of38kd and an isoelectric point of5.34.The identities of the amino acid sequence of the ANR gene from Vaccinium uliginosum compared with ANR genes from peach,apple,grape and wild strawberry were61%,62%,62%and59%,respectively.The phylogenetic tree constructed by amino acid sequence BLAST showed that the ANR gene of Vaccinium uliginosum was closely related to peach,apple and wild strawberry.