金黄色葡萄球菌L型对宫颈癌细胞生长的影响

Effect of staphylococcus aureus L-form on the growth of cervical cancer cells

目的:研究金黄色葡萄球菌(金葡菌)L型对体外培养的单层人宫颈癌细胞(He La)生长的影响作用。方法:用常规方法诱导金葡菌为L型后感染He La细胞,采用光学显微镜、透射电子显微镜检测金葡菌L型黏附及侵入细胞情况,并采用改良的MTT比色法检测细胞增殖能力。结果:光镜、电镜检查显示金葡菌L型能够黏附并且进入He La细胞;改良的MTT法检测表明金葡菌L型能够促进He La细胞增殖(P<0.01)。结论:金葡菌L型能够感染并促进He La细胞增殖生长,揭示金葡菌L型可能与宫颈癌的发生有关。

Objective:To observe the effects of Staphylococcus aureus L-form on the growth of human cervical cancer cells(HeLa)in vitro.Methods:The HeLa cells were infected with Staphylococcus aureus L-form by routine method.The adhesion and invasion of Staphylococcus aureus L-form were detected using light and electron microscopy,and the proliferation of HeLa cells was detected using improved MTT assay.Results:The results of light and electron microscopy L-form showed that the Staphylococcus aureus L-form could adhere and invade into HeLa cells.The result of improved MTT method showed that Staphylococcus aureus L-form could promote the proliferation of HeLa cells.Conclusions:Staphylococcus aureus L-form can infect and promote the growth of HeLa cells,which reveals that Staphylococcus aureus L-form may be one of inducting factor of cervical cancer.