寻常型银屑病患者血清IL-17、IL-23、IL-10、TGF-β水平及临床意义

Levels and significance of serum IL-17,IL-23,IL-10 and TGF-β in patients with psoriasis vulgaris

目的观察寻常型银屑病患者血清白细胞介素-17(IL-17)、白细胞介素-23(IL-23)、白细胞介素-10(IL-10)、转化生长因子-β(TGF-β)水平变化,探讨其在银屑病发病机制中的作用。方法选取82例寻常型银屑病患者为病例组,选取同期健康体检者40例为对照组。采用ELISA法检测两组血清IL-17、IL-23、IL-10和TGF-β水平,以皮损面积和严重程度(PASI)评分评估病情严重程度。结果病例组血清IL-17、IL-23水平高于对照组(P<0.05);病例组血清IL-10、TGF-β水平低于对照组(P<0.05);进行期和稳定期患者血清IL-17、IL-23水平高于对照组(P<0.05);进行期和稳定期患者血清IL-10、TGF-β水平低于对照组(P<0.05);银屑病患者血清IL-17水平与IL-23水平呈正相关(r=0.805,P<0.05);银屑病患者血清IL-10水平与TGF-β水平呈正相关(r=0.762,P<0.05);银屑病患者血清IL-17、IL-23水平分别与IL-10、TGF-β水平呈负相关(r=-0.731^-0.842,P均<0.05);银屑病患者血清IL-17、IL-23水平与PASI评分呈正相关(r=0.726、0.753,P均<0.05);银屑病患者血清IL-10、TGF-β水平与PASI评分呈负相关(r=-0.719、-0.778,P均<0.05)。结论血清IL-17、IL-23水平随寻常型银屑病的加重而上升,血清IL-10、TGF-β水平随寻常型银屑病的加重而下降,说明寻常型银屑病发生发展过程中发生了Th17/Treg细胞及相关细胞因子的失衡。

Objective To observe the change of serum interleukin-17(IL-17),interleukin-23(IL-23),interleukin-10(IL-10),and transforming growth factor-β(TGF-β)of patients with psoriasis vulgaris and explore its role in the pathogenesis of psoriasis.Methods82patients with psoriasis vulgaris were selected as the case group,and40healthy subjects were selected as the control group.The serum levels of IL-17,IL-23,IL-10and TGF-βwere detected by ELISA,and the severity of the disease was evaluated by skin lesion area and severity(PASI)scores in two groups.Results The serum levels of IL-17and IL-23in the case group were higher than those in the control group(P<0.05);The serum levels of IL-10and TGF-βin the case group were lower than those in the control group(P<0.05);The serum levels of IL-17and IL-23in the patients with progressive and stable phase were higher than those in the control group(P<0.05);The serum levels of IL-10and TGF-βin patients with progressive and stable phase were lower than those in the control group(P<0.05);The serum levels of IL-17were positively correlated with the level of IL-23in psoriasis patients(r=0.805,P<0.05);The serum levels of IL-10were positively correlated with the level of TGF-βin psoriasis patients(r=0.762,P<0.05);The serum levels of IL-17and IL-23were negatively correlated with the levels of IL-10and TGF-βin patients with psoriasis(r=-0.731^-0.842,P<0.05);The serum levels of IL-17and IL-23in patients with psoriasis were positively correlated with the PASI score(r=0.726,0.753,P<005);The serum levels of IL-10and TGF-βin patients with psoriasis were negatively correlated with the PASI score(r=-0.719,-0.778,P<0.05).Conclusion Serum IL-17,IL-23levels increased with the aggravation of psoriasis vulgaris,while serum IL-10,TGF-βlevels decreased with the aggravation of psoriasis vulgaris,suggesting the imbalance occurred in Th17/Treg cells and related cytokines in the onset and development of psoriasis vulgaris.