摘要
[目的]建立猕猴桃胚乳培养体系。[方法]将猕猴桃种子消毒后取胚乳作为外植体,接种于含有NAA和6-BA不同组合的1/2MS培养基上进行培养,调查不同NAA和6-BA组合对猕猴桃不同器官形成的影响。[结果]6-BA对不定芽的形成有促进作用,其中植物生长调节剂组合NAA 0.1μmol/L+6-BA 5μmol/L对不定芽的形成最有效,而植物生长调节剂组合NAA 0.5μmol/L+6-BA1μmol/L对不定根的形成最有效。[结论]该研究为猕猴桃的胚乳培养提供了技术支持。
[Objective]To establish in vitro culture system of kiwifruit endosperm.[Method]After disinfection of the seeds of kiwifruit,the endosperm was used as explant to inoculate on1/2MS medium containing different combinations of NAA and6-BA.We investigated the effects of different6-BA and NAA combinations on the organogenesis of kiwifruit endosperm callus tissue.[Result]6-BA preferably promote organogenesis of endosperm callus.The maximum induction frequency of the adventitious buds was observed in the combination of0.1jxmol/L NAA+5(xmol/L6-BA,while the maximum induction frequency of the adventitious roots was observed in the combination of0.5(xmol/L NAA+1|xmol/L6-BA.[Conclusion]This study provided technical support for kiwifruit endosperm culture.
作者
穆瑢雪
赵丹丹
刘永立
MU Roixue;ZHAO Dandan;LIU Yoili(College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, Zhejiang310058)
出处
《安徽农业科学》
CAS
2018年第1期59-60,共2页
Journal of Anhui Agricultural Sciences
基金
浙江省科技厅科技攻关重大农业项目“猕猴桃分子育种研究”(J31608)
关键词
猴桃
藤乳墙养
植犄生长调节剂
不定芽
不定根
Kiwifruit
Endosperm culture
Plant growth regulator
Adventitious buds
Adventitious roots
