摘要
以早熟砂梨品种鄂梨2号组培苗叶片为外植体,建立叶片再生体系。结果表明,鄂梨2号叶片再生不定芽最佳培养基为NN69+1.0 mg/L TDZ+0.1 mg/L IBA,愈伤组织再生率达到100%,不定芽再生率为36.67%;不定芽增殖最佳培养基为MS+1.0 mg/L 6-BA+0.2 mg/L IBA,增殖系数为2.19;不定芽在1 000mg/L ABT中浸蘸5 s后接种在添加500 mg/L活性炭的1/2MS培养基上,生根率最高,为66.67%;试管苗移栽成活率为84.44%。
Leaves excised from Eli2Hao were used as explants to establish regeneration system.The results indicated that the maximum adventitious buds regeneration frequency achieved36.67%on the mediu of NN69+1.0mg/L TDZ+0.1mg/L IBA.The most suitable medium for shoot multiplication was MS+1.0mg/L6-B A+0.2mg/L IBA and the average multiplication coefficient was2.19.The buds were dipped in1000mg/L ABT for5s and inoculated on1/2MS medium with500mg/L activated carbon.The highest rooting rate was66.67%,and the survival rate of plantlets was84.44%.
作者
涂俊凡
秦仲麒
李先明
伍涛
杨夫臣
朱红艳
刘政
杨立
TU Jun-fan;QIN Zhong-qi;LI Xian-ming;WU Tao;YANG Fu-chen;ZHU Hong-yan;LIU Zheng;YANG Li(Institute of Fruit and Tea, Hubtd Acadtmy of Agricultural Seienees/Fruit and Tea Branch,Hubei Innovation center of Agricultural Seienee and Technology, Wuhan 430064, China)
出处
《湖北农业科学》
2017年第23期4613-4615,共3页
Hubei Agricultural Sciences
基金
国家现代农业产业技术体系建设专项资金项目(CARS-28)
湖北省农业科技创新中心资助项目(2016-620-000-001-029)
湖北省科技支撑计划项目(2015BBA209)
国家科技支撑计划项目(2013BAD02B00)
湖北省农业科学院青年基金项目(2014NKYJJ16)
