摘要
设计合成了一种基于香豆素衍生物的水溶性荧光探针7-二乙氨基-3-甲醛香豆素,并通过~1HNMR,^(13)CNMR和MS确认其结构。探针具有良好的荧光发射性能,荧光最大发射峰位于471 nm;向其中加入Fe^(3+)后,荧光强度随着Fe^(3+)浓度的增加而逐渐减弱。探针L的荧光发射强度与Fe^(3+)的浓度在0.02~60.00μmol/L范围内呈良好的线性关系,可对Fe^(3+)进行定性与定量检测,检出限为22 nmol/L(S/N=3),对Fe^(3+)的选择性良好,不受其它常见金属离子的干扰。此外,探针对Fe^(3+)的检测具有可逆性。本探针具有很好的水溶性,在生理p H环境中检测效果较好成功用于人体淋巴肿瘤细胞Ramos中Fe^(3+)荧光成像。
A water-soluble fluorescent probe(7-diethyl amino-3-formaldehyde coumarin)for Fe3+detection was designed and synthesized,and its structure was confirmed by1HNMR,13CNMR and MS spectra.This probe showed high emission intensity at471nm.With the continuous addition of Fe3+,the emission intensities at471nm decreased gradually,and showed an excellent linearity with Fe3+in the range of0.02-60滋mol/L,and the regression equation was I=322.56-4.86CFe3+.This probe was able to detect Fe3+qualitatively and quantitatively with the detection limit as low as22nmol/L.Besides,this probe showed high sensitivity to Fe3+over other metal ions.The detection process was reversible,which could be recycled for the Fe3+detection.In terms of good optical properties and the strong fluorescence in physiological pH,the probe was successfully applied in imaging Fe3+of living Ramos cells.
作者
陈兆辉
李媛媛
韩娟
王赟
任彦鹏
陈桐
唐旭
倪良
CHEN Zhao-Hui;LI Yuan-Yuan;HAN Juan;WANG Yun;REN Yan-Peng;CHEN Tong;TANG Xu;NI Liang(School of Chemistry and Chemical Engineering Jiangsu University, Zhenjiang 212013, China;Jingjiang college Jiangsu University, Zhenjiang 212013, China;School of Food and Biological Engineering Jiangsu University, Zhenjiang 212013, China;Zhenjiang Entry-exit Inspection and Quarantine Bureau, Zhenjiang 212000, China)
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2018年第1期20-26,共7页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金项目(No.21576124)
江苏出入境检验检疫局科技计划项目(No.2016KJ51)
镇江市重点研发计划--社会发展项目(Nos.SH2015014
SH2016019)资助~~
关键词
荧光探针
铁离子
水溶性
细胞成像
Fluorescent probe
Ferric ion
Water solubility
Cell imaging