慢性心衰大鼠心肌miRNA-1、miRNA-133/Caspases表达差异研究

Differential Expression of miRNA-1, miRNA133/Caspases in Rats with Chronic Heart Failure

目的探讨慢性心衰大鼠心肌miRNA-1、miRNA-133/Caspases表达差异。方法 30只SD大鼠随机分为正常组10只,模型组20只。正常组等容量生理盐水腹腔注射,模型组予阿霉素腹腔注射造模;采用心脏彩超检测心功能,采用生理记录仪记录血流动力学,心脏切片行Tunel染色检测细胞凋亡,采用Real-time PCR法检测miRNA-1、miRNA-133、Caspase-3mRNA、Caspase-9mRNA表达;采用Western blot及免疫组化法检测心肌组织Caspase-3、Caspase-9蛋白表达水平。结果与正常组比,模型组大鼠左室舒张期内径(LVIDd)、左室收缩末期内径(LVESD)、左心室终末舒张压(LVEDP)明显增大(P<0.05),而左心室短轴缩短率(LVFS)、左室射血分数(LVEF)、心输出量(CO)、左心室平均压(LVAP)、左心室内压最大上升速率(dp/dtmax)及左心室内压最大下降速率(-dp/dtmax)下降(P<0.01),Tunel染色示心肌凋亡明显。miRNA-1、Caspase-3mRNA、Caspase-9mRNA、Caspase-3蛋白、Caspase-9蛋白表达增加(P<0.01),miRNA-133表达下降(P<0.01)。结论 miRNA-1、Caspase-3、Caspase-9在心肌凋亡中表达增加,miRNA-133表达下降。

Objective To investigate the different expression of miRNA1,miRNA133/Caspases in rats with chronic heart failure.Methods30SD rats were randomly divided into the normal control group(n=10),the model group(n=20).The normal group was injected intraperitoneally with equal volume normal saline,the model group was injected with doxorubicin intraperitoneally for modeling.The cardiac function was examed by color doppler ultrasound and hemodynamics was recorded.The cell apoptosis was determined by Tunnel staining method.The expression of miRNA-1,miRNA-133,Caspase-3mRNA,Caspase-9mRNA was determined by Real-time PCR,and the expression level of Caspase-3and Caspase-9in myocardial tissue of rats was detected by Western blot and immunohistochemistry.Results Compared with the normal group,the LVIDd,LVESD and LVEDP of rats in the model group increased significantly(P<0.05),while LVFS,LVEF,CO,LVAP,dp/dtmax and-dp/dtmax decreased(P<0.01).The myocardial apoptosis was obvious.The expression of miRNA-1,Caspase-3mRNA,Caspase-9mRNA,protein of Caspase-3and Caspase-9increased(P<0.01),and miRNA-133decreased(P<0.01).Conclusion The expression of miRNA-1,Caspase-3and Caspase-9increased and miRNA-133decreased in myocardial apoptosis.