大鼠尾椎椎间盘退变静态压力模型的构建

Construction of caudate degenerative intervertebral disc model by static pressure in rats

目的采用静态压力构建稳定的大鼠尾椎椎间盘退变模型。方法将40只12周龄大鼠随机分为静态压力组和针刺组,每组20只。静态压力组:在尾椎C_(4~7)椎体上安装静态压力环形外固定支架,压缩的4根弹簧施加的压强均为10 kPa,维持8周。针刺组:采用16 G针头刺入尾椎C_4/C_5/C_6/C_7椎间盘,限制损伤深度5 mm、朝向椎间盘中心,损伤后留置10 s。分别于术后8周进行组织病理学切片观察,采用秩和检验比较2组Thompson椎间盘退变病理分级。结果对2组大鼠尾椎共120个椎间盘髓核组织标本进行病理分级,统计结果显示静态压力组退变级别明显高于针刺组,差异有统计学意义(P<0.05)。结论采用静态压力构建的椎间盘退变模型稳定可靠,较传统针刺模型具有优势,可为临床椎间盘退变研究提供可靠的动物模型。

Objective To establish a stable rat caudate degenerative intervertebral disc model using static pressure.Methods Forty12-week-old rats were randomly divided into static pressure group and acupuncture group,with20rats in each group.In the static pressure group,the caudal vertebrae C4-7were intervened by10kPa static pressure for8weeks.In the acupuncture group,the caudal intervertebral discs C4/C5/C6/C7were acupunctured by a16G needle for10s.Histopathological sections were observed at8weeks after operation,and the Thompson disc degeneration pathological grading of the2groups was compared by rank-sum test.Results According to Thompson disc degeneration pathological grading,120rat caudal vertebrae in the2groups were graded,and the rank-sum test results showed that the static pressure group was superior to the acupuncture group(P<0.05).Conclusion The static pressure model is stable,reliable,and superior to the traditional acupuncture model,thus providing a reliable animal model for clinical research on disc degeneration.