瑞安市人民医院妇科门诊患者HPV亚型分布特点及其与女性宫颈病变的关系

Distribution of HPV subtype in gynecological outpatients in Ruian People′s Hospital and its relationship with female cervical lesions

目的了解浙江省瑞安市人民医院妇科门诊患者人乳头瘤病毒(HPV)总体感染情况及其型别分布特征,分析人乳头瘤病毒感染与宫颈病变的关系,为瑞安地区人乳头瘤病毒(HPV)感染及宫颈癌的预防及治疗提供初步建议。方法使用PCR-反向点杂交法,以2016年1月1日至2017年3月31日至瑞安市人民医院妇科门诊就诊的6631例患者为研究对象,对患者宫颈脱落细胞进行人乳头瘤病毒(HPV)基因分型检测。同时进行薄层液基细胞学检查(TCT),并对所得数据进行整理,分析妇科门诊患者人乳头瘤病毒(HPV)亚型分布特征及其与宫颈病变的关系。结果HPV总感染率为32.9%,其中高危感染率为24.3%,疑似高危型感染率为4.9%,低危型感染率为10.0%。HPV感染亚型前4分别是52型(7.5%)、53型(3.8%)、16型(3.5%)、58型(3.2%)。单一感染占71.1%,多重感染占28.9%。并且单一感染中以52型为主,多重感染中以52型合并感染为主。TCT检测结果显示ASC-US、ASC-H、LSIL、HSIL、SCC检出率分别为3.5%、0.9%、3.2%、0.6%和0.1%,而HPV感染检出率分别为68.1%、87.3%、90.6%、90.2%和100%。结论HPV52、HPV53、HPV16、HPV58在瑞安感染率最高,总体符合中国人群分布规律,但又具有明显地域特征。HPV感染可引起一系列宫颈病变,且基因型与宫颈疾病类型密切相关。

Objective To investigate the prevalence of human papillomavirus(HPV)infection and its type distribution in gynecological outpatients in Ruian People′s Hospital of Zhejiang Province,and to analyze the relationship between human papillomavirus infection and cervical lesions,and to provide preliminary suggestions for the prevention and treatment of human papillomavirus(HPV)infection and cervical cancer in Ruian.Methods A total of6631patients treated with gynecological outpatient department of Ruian People′s Hospital from January1,2016to March31,2017were used as the subjects to study the subtype of human papillomavirus(HPV)and perfom Thin-layer liquid-based cytology(TCT),and the data were analyzed.Then analys the distribution of human papillomavirus(HPV)subtype in gynecological outpatients and the relationship with cervical lesions.Results The total infection rate of HPV was32.9%,among which the high-risk type infection rate was24.3%,the suspected-high-risk infection rate was4.9%and the low-risk infection rate was10.0%.The first four subtypes were type52(7.5%),type53(3.8%),type16(3.5%),type58(3.2%).Single infection accounted for71.1%,multiple infection accounted for28.9%.The detection rates of ASC-US,ASC-H,LSIL,HSIL and SCC were3.5%,0.9%,3.2%,0.6%and0.1%respectively,and the detection rate of HPV infection was68.1%and87.3%,90.6%,90.2%,100%.Conclusion HPV52,HPV53,HPV16and HPV58have the highest infection rate in Ruian,which is consistent with the distribution of Chinese population,but has obvious regional characteristics.HPV infection can cause a series of cervical lesions,and genotype and cervical disease types are closely related.