生肌象皮膏对糖尿病溃疡大鼠Ang/Tie-2通路的影响

Effect of Shengji Xiangpi mastic on diabetic ulcer rats Ang/Tie-2 pathway

[目的]通过对糖尿病溃疡组织中酪氨酸激酶受体(Tie-2)、血管紧张素Ⅰ(Ang-1)及血管紧张素Ⅱ(Ang-2)mRNA转录水平的动态观察,探讨生肌象皮膏对糖尿病溃疡大鼠Ang/Tie-2通路的影响。[方法]首先应用链脲佐菌素(STZ)经大鼠尾静脉注射及背部打孔法制备2型糖尿病难愈性溃疡动物模型,按照血糖和体质量分层随机分为糖尿病对照组、生肌象皮膏组、凡士林组及空白对照组。生肌象皮膏组采用生肌象皮膏纱条于溃疡处外敷,凡士林组用凡士林纱条外敷,空白对照组、糖尿病对照组均以生理盐水纱条外敷,各组分别于创伤后1、3、7、14、21 d分别处死部分大鼠,并取溃疡组织,应用聚合酶链式反应(PCR)法检测各组大鼠溃疡组织中Ang-1、Ang-2和Tie-2 m RNA的转录水平。[结果]生肌象皮膏组各时间点肉芽组织中Ang-1、Tie-2蛋白表达水平均高于其余各组,Ang-2表达水平均低于其余各组(P<0.05)。[结论]生肌象皮膏可能通过增加创面肉芽组织中Ang-1,Tie-2的表达水平,抑制Ang-2表达方式,促进糖尿病大鼠溃疡面血管新生,加快糖尿病溃疡愈合。

[Objective]Through the dynamic observation of diabetic ulcers Tie鄄2,Ang鄄1 and Ang鄄2 transcriptional level,to investigate the effect of Shengji Xiangpi mastic on diabetic ulcer rats Ang/Tie鄄2 pathway.[Methods]The first step is having an injection of STZ at rats'tail vein and back drilling prepared ulcer refractory animal model of T2DM,according to blood glucose and body weight were randomly divided into diabetic group,saline group,Shengji Xiangpi mastic group and normal control group.Shengji Xiangpi mastic group used Shengji Xiangpi mastic gauze topical ulcer,vaseline group with vaseline gauze,control group,diabetic control group with saline gauze group,on the first day and in 3 days,7 days after trauma,14 days and 21 days respectively were part of rats.And take the ulcer tissue expression of Ang鄄1,Ang鄄2 and Tie鄄2 transcriptional level were detected by Western Blotting method in ulcer tissue.[Results]Shengji Xiangpi mastic group were higher than those of other groups of Ang鄄1 and Tie鄄2 transcriptional level at different time points in granulation tissue,the expression level of Ang鄄2 was lower than that of other groups(P<0.05).[Conclusion]Shengji Xiangpi mastic may through increase wound granulation tissue in Ang鄄1,the expression of Tie鄄2,inhibit the expression of Ang鄄2 in diabetic rats,and promote ulcer surface angiogenesis,accelerate the healing of diabetic ulcers.