芪术两活汤治疗痛风性关节炎实验研究

Experimental study of rat models with gouty arthritis treated by Qizhu Lianghuo decoction

[目的]探讨芪术两活汤水提液乙酸乙酯部位、乙醇部位对痛风性关节炎大鼠的作用。[方法]将90只SD大鼠随机分为9组:空白组、模型组、阿司匹林组、芪术两活汤水提液乙酸乙酯部位低、中、高剂量组及芪术两活汤水提液乙醇部位低、中、高剂量组。造模5 h后开始灌胃给药,观察各组大鼠关节炎症情况,连续给药7 d后取材,测定大鼠血清中尿酸(UA)的含量及血清白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)浓度,HE染色观察各组大鼠关节病理状态,并对单位面积炎症细胞计数。[结果]芪术两活汤水提液乙醇部位低、中、高剂量组与阿司匹林组在足趾炎症程度显著低于模型组(P<0.01);乙醇部位低、中、高剂量组及阿司匹林组大鼠血清UA水平及IL-1β、TNF-α浓度显著低于模型组(P<0.01,P<0.05);与模型组比较,芪术两活汤水提液乙醇部位低、中、高剂量组及阿司匹林组大鼠关节组织病理形态学状态均有显著改善(P<0.05),芪术两活汤水提液乙酸乙酯部位各剂量组与模型组比较,差异均无显著性(P>0.05);芪术两活汤水提液乙醇部位低、中、高剂量组及阿司匹林组大鼠关节组织炎症细胞计数显著低于模型组(P<0.05),乙酸乙酯部位组与模型组比较差异无显著性(P>0.05)。[结论]芪术两活汤水提液乙醇部位对痛风性关节大鼠模型具有抗炎作用。

[Objective]To explore the anti-inflammatory of the ethyl acetate fraction and the alcohol fraction of Qizhu Lianghuo decoction(QZLHD)extract on rat models with gouty arthritis.[Methods]Ninety male SD rats were randomly divided into nine groups(ten rats in each group):blank group,model group,aspirin group,ethyl acetate fraction of extracts of QZLHD groups(low-dose group,middle-dose group and high-dose group),alcohol fraction of extracts of QZLHD groups(low-dose group,middle-dose group,high-dose group).After5h of modeling,the inflammation of the joints of rats was observed.After7d of treatment,the concentrations of UA,IL-1βand TNF-αin serum were determined,the pathological morphology of the joint tissue were observed,and the numbers of inflammation cell per unit area were counted.[Results]The foot inflammation condition of alcohol fraction of QZLHD groups rats and aspirin group rats were significantly lower than those of model group(P<0.01).The concentrations of UA,IL-1βand TNF?鄄αfrom serum of alcohol fraction of QZLHD groups rats and aspirin group rats were significantly lower than those of model group(P<0.01,P<0.05).Compared with the model group,the pathological morphology of the joint tissue of rats of alcohol fraction of QZLHD groups and Aspirin group both have improved significantly(P<0.05),while those of the ethyl acetate fraction groups have no significant difference(P>0.05).The inflammatory cells among joint tissue in the alcohol fraction groups were significantly lower than that of model group(P<0.05)and there were no significantly difference between the ethyl acetate fraction groups and the model group(P>0.05).[Conclusion]The alcohol fraction of extracts of QZLHD has anti-inflammatory effects on rat models with gouty arthritis.