摘要
目的建立液相色谱串联质谱法(LC-MS/MS)同时检测血清中六大族类18种抗生素浓度的方法并对其进行方法学评价。方法采用Waters Oasis HLBμElution Plate固相萃取结合超高效液相色谱-串联质谱(SPE-LC-MS/MS)技术一次前处理和进样定量检测18种抗生素;参考化学药物临床药代动力学研究技术指导原则对方法进行特异性、标准曲线和定量范围、准确度和精密度等基本性能进行验证。结果特异性表明空白血清中的基质构成不影响目标抗生素的检测;标准曲线选取7个不同的浓度点定量,各浓度点的实测值与标示值之间最低浓度点的偏差在±20%以内,其余浓度点的偏差在±15%以内;添加回收率、准确度均在可接受范围内,RSD<15%。结论建立的SPE-LC-MS/MS方法的精密度、准确度、重现性均较好,结果准确,可用于临床实验室检测孕产妇血清中的目标抗生素残留。
Objective A UPLC-MS/MS analytical method was established and the concentrations of18kinds of antibiotics were detected.Method A total of18antibiotics were quantitatively analyzed by solid-phase extraction(Waters Oasis HLB^Elution Plate)combined with UPLC-MS/MS.Reference to the clinical pharmacokinetics of chemical drugs guidelines,the specificity,standard curve,quantitative range,accuracy and precision of the method were verified.Results the specificity showed that the matrix composition in blank serum did not affect the detection of target antibiotics.Seven different concentrations were selected in the standard curve.The deviation between the measured value and marked value was within±20%,and the deviation of the remaining concentration iswithin±15%.The fortified recovery and accuracy is acceptable,RSD<15%.Conclusions The accuracy,precision and reproducibility of the established SPE-LC-MS/MS method were satisfactory and the results were accurate and the method could beused to detect antibiotics residues in serum in clinical laboratory.
出处
《中国产前诊断杂志（电子版）》
2017年第4期32-37,共6页
Chinese Journal of Prenatal Diagnosis(Electronic Version)
关键词
固相萃取
夜相串联质谱
抗生素
solid-phase extraction liquid chromatography-tandem mass spectrometry
antibiotic
