蓖麻二倍体与同源四倍体光合特性和逆境生理指标的比较研究

Comparative Studies on Photosynthetic Characteristics and Stress Physiological Indexes Between Diploid and Autotetraploid in Ricinus communis L.

【目的】评价同源四倍体蓖麻的育种意义。【方法】以二倍体和同源四倍体蓖麻为材料,测定光合速率,过氧化物酶(POD)、超氧化物歧化酶(SOD)活性,及叶绿素、可溶性糖、可溶性蛋白、游离脯氨酸(Pro)、丙二醛(MDA)等含量,并比较上述指标的种间差异。【结果】同源四倍体蓖麻的叶绿素含量、净光合速率、叶片可溶性糖含量、可溶性蛋白含量、游离脯氨酸含量、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性均显著地高于二倍体,分别提高30.00%、53.47%、19.23%、25.22%、11.72%、262.07%和24.20%,丙二醛(MDA)的积累明显低于二倍体,降低30.57%。【结论】四倍体蓖麻可为蓖麻种质资源提供较宝贵新种质。

【Objectives】In order to evaluate the breeding significance of autotetraploid castor.【Method】comparative studies were carried out on photosynthetic characteristics and stress physiological indexes between diploid and autotetraploid in castor in this paper.【Result】The chlorophyll content,the net photosynthetic rate,the soluble sugar content,the soluble protein content,the free proline content,the peroxidase(POD)and superoxide dismutase(SOD)activity in leaves of the autotetraploid were significantly higher than those in diploid,increased by30%,53.47%,19.23%,25.22%,11.72%,262.07%and24.20%respectively.Meanwhile the malondiadehyde(MDA)accumulation in autotetraploid was significantly reduced by30.57%.【Conclusion】Tetraploid castorprovides valuable new germplasm for germplasm resources of castor.