基于食用油为连续相的茶多酚反相微乳液的制备

Preparation of Tea Polyphenols-Loaded Inverse Microemulsion Using Edible Oil as Continuous Phase

制备油烯基聚氧乙烯(10)醚(polyoxyethylene(10)oleyl ether,Brij97)-单油酸甘油酯(glyceryl monooleate,GMO)-乙醇-食用油-水反相微乳液,成功将抗氧化剂茶多酚添加至食用油中。通过拟三元相图法、动态光散射法和差示扫描量热法研究微乳液的相行为、粒径和抗氧化能力。结果显示,微乳液的最佳配方条件为复合表面活性剂Brij97与GMO质量比2∶8、水相中水与乙醇质量比4∶1。微乳液中茶多酚质量浓度越高,微乳液粒径越小,但多分散指数越大。当水相中茶多酚质量浓度达到0.10 g/m L时,30 d内5种微乳液粒径都稳定在8.00~12.00 nm范围内。差示扫描量热法显示空白大豆油微乳液的起始氧化温度为163.40℃,添加0.01 g/m L茶多酚后为175.77℃,添加0.10 g/m L茶多酚后为210.88℃。

Tea polyphenols-loaded water-in-oil microemulsion(ME)of polyoxyethylene(10)oleyl ether(Brij97)-glycerylmonooleate(GMO)-ethanol-edible oil-water to envelop was prepared successfully in this paper.The phase behavior,particle size,and oxidative stability were investigated by pseudo-ternary phase diagram method,dynamic light scattering(DLS)and differential scanning calorimetry(DSC).The results showed that the optimal mass ratio of Brij97to GMO was2:8;the optimal mass ratio of water to ethanol was4:1.Higher tea polyphenol(TP)concentration resulted in smaller mean particle size and higher polydispersity index of ME system.Moreover,the mean particle size of5kinds of ME systems with0.10g/mL TP in the aqueous phase remained between8.00and12.00nm for30days.The results of DSC showed that the incipient oxidation temperature of blank soybean oil ME was163.40℃,while those of ME with0.01and0.10g/mL TP added were175.77and210.88℃,respectively.