胁迫生长条件下副溶血性弧菌的生物特性分析

Biological Characteristics of Vibrio parahaemolyticus during Growth in Adverse Environment

为了解副溶血性弧菌在不同环境应激条件下的生长情况,以副溶血性弧菌标准菌株(Vibrio parahaemolyticus,CICC21617)为研究对象,使其在低盐度(0.9 g/100 m L NaCl)、低pH值(pH 5.0)和高温(50℃)条件下适应性传代培养后,通过测定菌株生存能力、生物被膜生成能力、溶血素活力和胞外蛋白酶产量等指标分析胁迫条件下副溶血性弧菌的生物特性。结果显示,经过胁迫传代的菌株较原始菌株,其生存能力和生物被膜生成能力有所增强,而溶血素活力和胞外蛋白酶产量却明显下降,外膜蛋白的种类和表达量亦有所不同。结果说明副溶血性弧菌可能是通过改变自身生物学特性来适应外界的不利生存环境,且菌株经应激适应后对逆境生存环境的耐受性明显增强。

The objective of this study was to observe the growth characteristics of Vibiro parahaemolyticus under various environmental stresses.The survival,biofilm formation ability,hemolysin activity and extracellular protease production of V.parahaemolyticus(CICC21617)exposed to low salinity(0.9g/100mL NaCl),low pH(5.0)or high temperature(50℃)were investigated after10passages.It was found that the stressed and passaged strains had enhanced survivaland biofilm formation ability and decreased hemolysin activity and showed significantly reduced extracellular protease production compared with the parental strain.The expression levels and types of low molecular weight outer membrane proteins of the stressed strains were also different between the parental and passaged strains.Thus,V.parahaemolyticus is capable of changing their own biological characteristics to adapt to adverse conditions.Furthermore,the tolerance of V.parahaemolyticus to environmental stress can be enhanced after adaptive training.