石墨烯量子点荧光猝灭-恢复法测定Cu^(2+)与谷胱甘肽的含量

Detection of copper ions and glutathione based on off-on fluorescent graphene quantum dots

通过在温和条件下氧化石墨粉的方法合成高分散性、高荧光强度的石墨烯量子点(GQDs),并运用此GQDs对Cu^(2+)和GSH具有荧光猝灭-恢复的效应,建立对这两种物质简便、快捷的检测方法。Cu^(2+)与GSH的浓度分别在1.0~10.0 mmol/L、0.1~1.0 mmol/L范围内与GQDs的荧光强度呈良好线性关系,检测限分别为0.01和0.1 mmol/L。此外,在实际样品的检测中,加标法测得的回收率分别为93%~101%、96%~107%。该方法操作简便,测定准确,精密度高,且常见的金属离子及潜在共存物质对Cu^(2+)与GSH的检测均没有干扰。

We developed a rapid method to detect Cu2+and glutathione(GSH)based on the fluorescence quenching-recovery properties of graphene quantum dots(GQDs),which were synthesized by the gentle oxidation of graphite powder.The results revealed that the fluorescence intensity of GQDs versus concentration of Cu2+and GSH had good linearity,with the detection limits of0.01and0.1mmol/L,respectively.The fluorescence quenching was linearly proportional to the concentrations of Cu2+ranging from1.0to10.0mmol/L;the fluorescence intensity was linearly enhanced with the concentrations of GSH ranging from0.1to1.0mmol/L.In addition,the method was successfully applied to the determination of real samples with recoveries falling between93%-101%and96%-107%,respectively.This method is simple,accurate and precise.There was no interference with other familiar co-existing metal ions and potential materials.