白花蛇舌草通过JAK2/STAT3通路途径诱导膀胱癌T24细胞凋亡机制

Herba Hedyotis Diffusae induces human bladder cancer T24 cells apoptosis through inhibiting JAK2/STAT3 signaling pathways

目的研究白花蛇舌草通过JAK2/STAT3通路途径诱导膀胱癌T24细胞凋亡的机制。方法将人膀胱癌T24细胞接种到培养液中,使用MTT法对1 mg/kg、2 mg/kg、5 mg/kg不同浓度的白花蛇舌草对T24增殖的影响进行检测,使用双标记流式细胞术对细胞的凋亡率进行检测,使用Western blot对细胞中的JAK2及STAT3的表达情况进行检测。结果白花蛇舌草能够抑制膀胱癌T24细胞增殖,并且还能够诱导T24细胞的凋亡,其诱导作用随着白花蛇舌草浓度增加而增加,JAK2及STAT3随着白花蛇舌草浓度的增加而表达水平有所降低。结论白花蛇舌草能诱导膀胱癌T24细胞凋亡,可能和JAK2/STAT3通路有密切的联系。

Objective To study the mechanism of induces human bladder cancer T24cells apoptosis through inhibiting JAK2/STAT3signaling pathways.Methods Human bladder cancer T24cells were inoculated into the culture medium,MTT assay was used to evaluate the effect of1mg/kg,2mg/kg and5mg/kg different concentrations of Herba Hedyotis Diffusae induces human bladder cancer T24cells;the expression of JAK2and STAT3in cells were detected by Western blot.Results Herba Hedyotis Diffusae induces human bladder cancer T24cells apoptosis according to the results of MTT.Herba Hedyotis Diffusae can inhibit the expression of JAK2,and STAT3by the detection of Western blot Methods.Conclusion Herba Hedyotis Diffusae induces human bladder cancer T24cells apoptosis through inhibiting JAK2/STAT3signaling pathways.