泽泻汤加味方对高脂血症模型大鼠肝组织中水通道蛋白8的影响

Effects of Modified Rhizoma Alismatis Decoction on the Expression of AQP8 in Liver Tissue of Hyperlipidemia Model Rats

目的:观察泽泻汤加味方对高脂血症模型大鼠肝组织中水通道蛋白8(AQP8)表达的影响,探讨该方防治高脂血症的机制。方法:将60只大鼠随机分为空白对照组(蒸馏水)、模型组、阳性对照组(辛伐他汀1.89 mg/kg)和泽泻汤加味方高、中、低剂量组(29.56、14.78、7.39 g/kg,以生药量计),每组10只。除空白对照组大鼠给予正常饮食外,其余各组大鼠均给予高脂饲料以复制高脂血症模型,并于造模同时每天灌胃给药1次,连续给药5周。给药结束后,检测大鼠血清中三酰甘油(TG)、胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)含量,观察大鼠肝组织病理形态学变化,并检测大鼠肝组织中AQP8 m RNA和蛋白表达水平。结果:与空白对照组比较,模型组大鼠血清中TG、TC、LDL-C含量显著升高(P<0.05或P<0.01),血清中HDL-C含量显著降低(P<0.01),肝组织出现细胞排列不整齐、肝血窦充血水肿等病理学变化,肝组织中AQP8 m RNA和蛋白表达水平显著升高(P<0.01);与模型组比较,各给药组大鼠上述指标均显著改善(P<0.05或P<0.01),肝组织的结构趋于正常、脂肪变性明显减轻。结论:泽泻汤加味方可能通过下调肝组织中AQP8 m RNA及蛋白的表达,发挥其对高脂血症的防治作用。

OBJECTIVE:To observe the effects of Modified Rhizoma Alismatis decoction on the expression of aquaporin 8(AQP8)in liver tissue of hyperlipemia model rats,and to investigate the mechanism of preventing and treating hyperlipemia.METHODS:Total of 60 rats were randomly divided into blank control group(distilled water),model group,positive control group(simvastatin 1.89 mg/kg)and modified Rhizoma Alismatis decoction high-dose,medium-dose and low-dose groups(29.56,14.78,7.39 g/kg,calculated by crude drug),with 10 rats in each group.Those groups were given high-fat diet to induce hyperlipemia model and given relevant medicine intragastrically once a day for consecutive 5 weeks except that blank control group was given normal diet.After administration,the serum contents of TG,TC,HDL-C and LDL-C in rats were detected,and the pathomorphology changes of liver tissue were observed;the mRNA and protein expression of AQP8 in liver tissue were detected.RESULTS:Compared blank control group,the serum contents of TG,TC and LDL-C in model group were increased significantly(P<0.05 or P<0.01),while the serum content of HDL-C was decreased significantly(P<0.01);pathological changes were found in liver tissue,such as irregular cell arrangement and hepatic sinusoidal hyperemia and edema;mRNA and protein expression of AQP8 in liver tissue were increased significantly(P<0.01).Compared with model group,above indexes of treatment groups were improved significantly(P<0.05 or P<0.01);the structure of liver tissue tended to be normal and the fatty degeneration was obviously alleviated.CONCLUSIONS:Modified Rhizoma Alismatis decoction can regulate the mRNA and protein expression of AQP8 in liver tissue so as to play the effects on the prevention and treatment of hyperlipidemia.