CXCL10与CXCL12基因多态性与肺结核易感性的关系

Association of gene polymorphism of CXCL10 and CXCL12 with tuberculosis susceptibility

目的探讨趋化因子CXCL10-135G/A和CXCL12-801G/A的基因多态性与肺结核病易感性之间的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对102例结核病患者(病例组)和115例健康对照者(对照组)的CXCL10-135G/A和CXCL12-801G/A多态性进行基因分型,分析这两个位点多态性与结核病发生是否相关。结果病例组和对照组CXCL10-135G/A与CXCL12-801G/A基因型分布符合Hardy-Weinberg平衡定律。CXCL10-135G/A多态性基因型及等位基因的分布频率在病例组及对照组中差异均有统计学意义(均P<0.05),病例组G等位基因的分布频率高于对照组,A等位基因的分布频率低于对照组。CXCL12-801G/A多态性基因型及等位基因的分布频率在病例组及对照组中差异均无统计学意义(均P>0.05)。结论趋化因子CXCL10-135G/A基因多态性与肺结核易感性有关,而CXCL12-801G/A基因多态性与肺结核感染可能无关。

Objective To explore the relationship of chemokines CXCL10-135G/A and CXCL12-801G/A gene polymorphisms with susceptibility to tuberculosis.Methods CXCL10-135G/A and CXCL12-801G/A polymorphisms of102tuberculosis patients(case group)and115healthy controls(control group)were genotyped by polymerase chain reactionrestriction fragment length polymorphism(PCR-RFLP),and the relationship between the two polymorphisms and susceptibility to tuberculosis were analyzed.Results The genotype analysis of CXCL10-135G/A and CXCL12-801G/A was in accord with the law of Hardy-Weinberg equilibrium in the case group and the control group.The differences of genotype and allele distribution frequency of CXCL10-135G/A were statistically significant between the case group and the control group(all P<0.05).The frequency of G allele distribution was higher in the case group than that in the control group,and the frequency of A allele distribution was lower than that in the control group.There were no significant differences in genotype and allele distribution frequency of CXCL12-801G/A polymorphism between the case group and the control group(all P>0.05).Conclusion Chemokine CXCL10-135G/A gene polymorphism is associated with susceptibility to pulmonary tuberculosis,and CXCL12-801G/A gene polymorphism may not be associated with tuberculosis infection.