动物挤压伤全真模拟平台的建立及效果初步评价

The establishment of animal crush injury simulation platform and its preliminary evaluation

目的应用全真地震模拟振动平台对动物进行挤压伤造模,观察动物存活率及挤压综合征发生率,评价构建稳定、高操控性、全真模拟地震环境的挤压伤平台造模效果。方法依托灾害救援医学全军重点实验室,联合北京维尔森科技发展有限公司,设计并研发全真地震模拟振动平台,并在平台上搭建等比缩尺建筑,选用30只Wistar大鼠,随机分为6组,对照组及实验1~5组,每组5只,分次置于缩尺建筑物内进行地震致挤压伤造模,观察挤压综合征发生率及大鼠存活率,以评价平台挤压效果。结果全真地震模拟振动平台上搭建稳定的等比缩尺建筑,可将大鼠放于建筑物中,进行地震致挤压伤全真还原模拟实验,所有实验组大鼠(n=25),解除埋压时的存活率为96%(24/25);解除埋压后24 h的存活率为80%(20/25);肌红蛋白尿出现率为24%(6/25),其中1只出现无尿。结论研制出一种操控性强、可多次倒塌的全真模拟地震伤动物实验模型,该模型可作为地震致动物挤压伤模型的全真模拟震动平台,为开展一系列动物挤压伤相关研究提供基础平台,但其稳定性尚需要进一步大量动物造模验证。

Objective The objective of this study was to make a model by using a real earthquake simulation vibrating platform to crush the animals,to observe their survival rate and the incidence of crush syndrome,and to assess the effect of building a stable,highly manipulative,and full simulation platform where the earthquake crushes develop.Methods This study was supported by the Key Laboratory of Disaster Rescue Medicine and Beijing Wilson Technology Development Co.,Ltd,by whom a real earthquake simulation vibrating platform was designed and scale models was built on it.A total of30Wistar rats were randomly divided into one control group and five experimental groups,with5rats in each group.They were placed in batches in scale buildings for producing a crush injury model.The crush syndrome incidence and survival rate of rats was recorded and the effect of crushing was evaluated.Results Establishing a stable geometric scale building on earthquake simulation platform could enable reproducing earthquake crush injury by placing rats in the building.The survival rate of all the rats in the experimental groups(n=25)when relived from burying was96%(24/25),and decreased to80%(20/25)after24hours.The incidence of myoglobinuria was24%(6/25),and only one of them had no urine.Conclusions A highly maneuverable and collapse-resistant simulation earthquake model can serve as the stimulation platform where the earthquake crushes develop and provide the basic platform for the research of a series of animal crush injuries.Of course,the stability of the model still needs to be verified by a large number of animal models.