水稻锌铁转运蛋白ZIP基因家族研究进展

Progress in ZIP transporter gene family in rice

锌(zinc,Zn)和铁(iron,Fe)是水稻(Oryza sativa L.)生长必需的矿质元素,也是人体必需的微量元素。水稻体内Zn、Fe含量维持在适宜水平有利于提高其产量和品质,提高稻米中Zn、Fe含量能够在一定程度上解决人体Zn、Fe营养缺乏的问题。因此,研究水稻中Zn和Fe等微量元素转运蛋白的具体功能对于提高水稻产量和稻米品质具有重要意义。锌铁转运蛋白(zinc-regulated transporters and iron-regulated transporter-like protein,ZIP)负责Zn和Fe等离子的吸收、转运和分配,是维持水稻中Zn和Fe平衡的重要转运蛋白,其表达水平受Zn和Fe水平影响。ZIP基因家族在自然群体中具有丰富的等位变异,而且某些单倍型存在明显的籼粳分化,这可能造成了不同品种间籼、粳稻中Zn和Fe积累的差异。目前,已有大量关于ZIP基因家族的研究,但只有Os ZIP3的作用机制研究的较为清楚。另外,对Zn、Fe在籽粒中的积累机制研究和自然群体中ZIP基因的等位变异研究还不够深入。因此,ZIP转运蛋白家族仍存在较大的研究空间。本文详细介绍了ZIP转运蛋白在水稻体内的亚细胞定位、表达模式、转运机制以及在自然群体中的等位变异等,以期为研究水稻稻米微量元素的积累提供理论基础,为提高稻米品质提供借鉴。

Zinc and iron are essential mineral elements for the growth of Oryza sativa L.and also micronutrients for human health.Therefore,it is vital to study biofortification of rice with Zn and Fe in order to improve the yield and quality of rice,as well as to enhance nutritional states of humans.The zinc-regulated transporters and iron-regulated transporter-like proteins(the ZIP family)control the absorption and translocation of Zn and Fe and maintain their homeostasis in rice.Reciprocally,the expression of the ZIP family is induced by the concentration of Zn and Fe.There are abundant natural allelic variations of the ZIP genes,and some haplotypes only occur in indica or japonica,which could affect Zn and Fe accumulation levels between these subspecies.Currently,emerging functional studies of the accumulation mechanism of Zn and Fe in grains reveal that a lot still needs to be learned about the allele variations of ZIP genes.In fact,only OsZIP3is functional characterized.In this review,we summarize the latest progress in the molecular characteristics of the ZIP transporters,including protein localization,gene expression patterns,transport mechanism,metal ion interaction,and natural allelic variations.