大连市万寿菊叶斑病病情调查及病原菌鉴定初报

Preliminary report on the investigation and identification of Marigold leaf spot pathogens

万寿菊(Tagetes erecta L)具有较高的观赏价值和药用价值,是城市园林中常见的草本花卉.因其具有特殊气味,栽培中病害少有发生.但近几年,大连市园林中的万寿菊叶斑病发生较重,查明病因、为病害防治提供理论依据尤为重要.采用单斑分离、致病性检测、形态学及分子生物学鉴定,查明了大连市万寿菊叶斑病的病原菌为2种真菌,分别为链格孢属的细交链孢霉(Alternaria tenuissima)和球黑孢霉菌属的球黑孢(Nigrospora sphaerica).球黑孢侵染万寿菊,导致其发生叶斑病鲜有报道.

Marigold(Tagetes erecta L)is a common herbaceous flower in urban landscape with a high ornamental and medicinal value.Because of its special smell,rare occurrence of disease in cultivation.However in recent years,serious leaf spot appears in Marigold of Dalian.Therefore,it is imperative to find out the pathogenesis and provide theoretical basis for the disease prevention.To identify the pathogens of the leaf spot of Marigold,in this paper,several methods were used such as isolation pathogens from the single spot,pathogenicity test,morphological and molecular biology identification,etc.The results show that Alternaria tenuissima and Nigrospora sphaerica are two kinds of pathogens that lead to the Marigold leaf spot in Dalian.So far,N.sphaerica of the Marigold leaf disease is rarely reported