物体认知——基于动物自发行为的一种学习记忆评价方法的研究

Object recognition test—a method to assess the learning and memory in mice based on their spontaneous behavior

目的研究物体认知实验方法中的新物体识别实验、物体位置识别实验、时序记忆实验和情景记忆实验四种模式中实验步骤和评价指标,为学习记忆研究提供一种基于动物自发行为,多模式、精细敏感的行为实验方法。方法选择KM和ICR两种品系小鼠,采用四种实验模式,研究其测试期、熟悉期及间隔时间等。结果四种实验模式中,熟悉期5 min,熟悉期与测试期间隔10 min,测试期为3~5 min时可较好地评价动物记忆能力。结论本研究系统明确了物体认知四种模式的检测步骤、间隔时间和评价指标,为物体认知用于学习记忆障碍发生发展机制、新药和保健品研发提供了稳定可靠、规范的操作方法。

Objective To develop a multi-mode,detailed and sensitive object recognition method for learning and memory research,including novel object recognition task,object location recognition task,temporal order memory task and object context recognition task in animals based on their spontaneous behavior.Methods Two strains of mice(KM and ICR)and four patterns were used to establish the object recognition method and several relative factors,such as time of test phase,delay between test phase and familiar phase were investigated.Results It was stable and reasonable to set the time of familiar phase as 5 min,the interval between test phase and familiar phase as 10 min,and the time of test phase as 3-5 min,under the precondition of habituation for three days and selecting the appropriate object pairs.Conclusions Object recognition test including four patterns has been systematically established as a stable,reliable and normative way for the research of animal learning and memory and drug development.