孤养对单次延长应激建立的创伤后应激障碍模型大鼠行为学的影响

Effect of social isolation on the behavior of rat models of post-traumatic stress disorder established by single-prolonged stress

目的通过单次延长应激(SPS)建立创伤后应激障碍(PTSD)模型,观察孤养对SPS模型大鼠的影响。方法将36只大鼠随机分为正常组、SPS模型组及SPS联合孤养组,SPS模型组、SPS联合孤养组给予SPS造模,SPS联合孤养组造模后进行单独饲养。造模7 d后检测大鼠体重增长数,旷场实验中运动总距离、穿格次数以及单次最大运动距离,僵立行为测试中僵立行为次数及时间。结果与正常组大鼠进行比较,SPS模型组大鼠体重增长数、单次最大运动距离明显减少(P<0.01),运动总距离、穿格次数减少(P<0.05),僵立行为测试僵立行为次数及时间明显增加(P<0.01);SPS联合孤养组大鼠体重增长数减少(P<0.05),僵立行为测试僵立行为次数及时间增加(P<0.05)。与SPS模型组比较,SPS联合孤养组旷场实验中运动总距离、穿格次数以及单次最大运动距离增加(P<0.05)。结论本实验成功通过单次延长应激复制了创伤后应激障碍大鼠模型,7 d孤养使得SPS模型焦虑状态得到改善。

Objective To establish a rat model of post-traumatic stress disorder(PTSD)through single-prolonged stress(SPS)and to observe the effect of social isolation on the behavior of the SPS model rats.Methods A total of 36 rats were randomly divided into the normal group,SPS model group and SPS combined with social isolation group.The rats in both SPS model group and SPS combined with social isolation group were modeled by single-prolonged stress,and the rats in the SPS combined with social isolation group were raised separately after modelling.The weight gaining,the total movement distance in open field test,the frequency of grid crossing,the single maximum movement distance,and the freezing frequency and time durations in the freezing behavior test were measured after 7 days of modeling.Results Compared with the normal group,the weight gaining and the single maximum movement distance of the rats in the SPS model group were significantly decreased(P<0.01),as well as the total movement distance and the frequency of grid crossing(P<0.05),while the freezing frequency and time in the freezing behavior test were significantly increased(P<0.01).Compared with the normal group,the weight gaining and crossing times of the rats in the SPS combined with social isolation group was decreased(P<0.05),and the freezing frequency and time durations in the freezing behavior test were increased(P<0.05).In addition,compared with the SPS model group,the total movement distance in the open field test,the frequency of grid crossing and the single maximum movement distance of the rats in the SPS combined with social isolation group were increased(P<0.05).Conclusions The rat model of post-traumatic stress disorder is successfully established by single-prolonged stress,and 7 days of social isolation may alleviate the anxiety state of SPS model rats