烟草胞质雄性不育系与保持系内源植物激素水平差异性研究

Differences of Endogenous Plant Hormone Level in Tobacco between Cytoplasmic Male Sterile Line and Maintainer Line

[目的]研究烟草包质雄性不育系与其保持系生长期内源植物激素含量的变化规律和激素水平差异。[方法]选取K326、K394和云烟87共3组不育系与保持系为研究材料,取苗期、团棵期、旺长后10 d、现蕾期(叶和花蕾)、中心花开放期(叶和花蕾)的功能叶片和花蕾,采用高效液相色谱法测定其各时期生长素(IAA)、赤霉素(GA)、脱落酸(ABA)、N6-异戊烯腺嘌呤(i PAs)和反玉米素核苷(ZRs)5种植物内源激素含量。[结果]保持系与不育系的IAA含量比值各时期叶片中均大于1,而花蕾小于1。保持系与不育系的ABA含量比值各时期叶片和花蕾中均大于1。保持系与不育系的i PAs含量比值各时期叶片中均小于1,花蕾中大于1。现蕾期保持系和不育系的ZRs+GA比值小于1,其他各时期规律不明显。[结论]该研究为更好地应用烟草的雄性不育现象提供理论依据。

[Objective]To study the difference of hormone level and change rule and the difference of endogenous phytohormones in tobacco male sterile line and its maintainer line at growth stage.[Method]We selected three treatments of male sterile lines and maintiainer lines(K326,K394and Yunyan87)as the research materials,and adopted functional leaves and buds of seven different growth stages(seedling stage,rosette stage,prosperous growth for10d,squaring stage and central flower opening stage).Five endogenous hormone contents(IAA,GA,ABA,iPAs,ZRs)of sample from seven different growth stages were determined by high-performance liquid chromatogram.[Result]IAA content ratio of maintainer line to male sterile line was more than1in leaves in different growth stages,but those in buds were smaller than1.ABA content ratio of maintainer line to male sterile line was more than1in different growth stages.iPAs content ratio of maintainer line to male sterile line in leaves was smaller than1in different growth stages,but those in bud greater than1.ZRs+GA content ratio of maintainer line to male sterile line in squaring stage was less than1.The laws of other periods were not obvious.[Conclusion]This research provided theoretical basis for the application of male sterility of tobacco.