栓叶野茉莉种子萌发生理特性研究

Study on Physiological Characteristics during Seed Germination of Styrax suberifolius

[目的]研究栓叶野茉莉(Styrax suberifolius Hook.et Arn.)种子萌发生理特性。[方法]测定栓叶野茉莉种子发芽率及种子萌发过程中内含营养物质及相关酶活性的动态变化。[结果]栓叶野茉莉种子发芽率在置床后15 d迅速升高,在整个发芽过程中呈"S"形变化,最终达到18.5%;种子内可溶性糖含量呈先升后降的趋势,与淀粉含量恰好相反;可溶性蛋白含量在整个萌发过程中不断上升,最终达到256.18 mg/g(FW);α-淀粉酶和β-淀粉酶活性在置床后20 d内保持较高水平,随后大幅下降;过氧化物酶活性则呈现出单峰特性,在第10天达到最大值。[结论]较高的淀粉酶与过氧化物酶活性有利于栓叶野茉莉萌发过程中淀粉水解及可溶性蛋白的积累。

[Objective]To study on physiological characteristics during seed germination of Styrax suberifolius.[Method]The germination percentage and changes of seed reserve contents as well as certain enzyme activities of Styrax suberifolius seeds during the process of germination were determined.[Result]The results showed that the germination percentage climbed rapidly after sowing for15days,and it had the“S”curve before the germination percentage finally reached18.5%;Meanwhile,the soluble sugar content showed a up-and-down changing pattern,while the starch performed oppositely;The soluble protein content progressively increased during the seed germination,reaching256.18mg/g FW;α+βamylase activities remained high at the first20days,and then dropping massively;POD activity peaked at10days after sowing.[Conclusion]The relatively high amylase and POD activity helped the degradation of starch and the accumulation of soluble protein in S.suberifolius seeds during germination.