摘要
目的:研究配对相关同源框2(paired-related homeobox 2,PRRX2)基因对胃癌细胞活力和迁移能力的影响,并分析其调控Wnt/β-catenin信号通路的机制。方法:通过生物信息学分析在线数据库中胃癌和正常胃组织的PRRX2表达水平及PRRX2在胃癌组织中的表达与胃癌患者总生存率的相关性;将PRRX2小干扰RNA(si RNA)和过表达质粒分别转染到胃癌细胞MGC-803和SGC-7901中,敲低和过表达PRRX2基因;MTT法和Transwell实验检测胃癌细胞的活力和迁移能力;Western blot和TOPflash/FOPflash双萤光素酶报告基因实验检测Wnt/β-catenin信号通路的活化情况;免疫共沉淀实验检测PRRX2与β-catenin蛋白的相互作用。结果:敲低PRRX2能够减弱胃癌细胞MGC-803的活力和迁移能力(P<0.05)。过表达PRRX2能够增强胃癌细胞SGC-7901的活力和迁移能力(P<0.05),增加β-catenin、c-Myc和cyclin D1的蛋白的表达水平(P<0.05),增强TOPflash/FOPflash双萤光素酶报告基因活性(P<0.05)。PRRX2与β-catenin蛋白存在相互作用。结论:PRRX2可促进胃癌细胞的活力和迁移,其机制可能与Wnt/β-catenin信号通路有关。
AIM:To study the effect of paired-related homeobox2(PRRX2)gene on the viability and migration ability of gastric cancer cells,and to analyze the underlying mechanism of regulating Wnt/β-catenin signaling pathway.METHODS:The expression of PRRX2in gastric cancer and normal gastric tissue and the correlation between PRRX2expression in gastric cancer tissues with the overall survival rate of gastric cancer patients were analyzed by bioinformatics.The small interfering RNA(siRNA)and over-expressed plasmids of PRRX2were transfected into gastric cancer cells MGC-803and SGC-7901,respectively.MTT assay and Transwell assay were used to detect the viability and migration ability of gastric cancer cells.Western blot and TOPflash/FOPflash dual-luciferase reporter gene assay were used to detect the activity of Wnt/β-catenin signaling pathway.Co-immunoprecipitation was used to detected the interaction between PRRX2andβ-catenin proteins.RESULTS:Knockdown of PRRX2attenuated the viability and migration ability of gastric cancer cell line MGC-803(P<0.05).Over-expression of PRRX2enhanced the viability and migration ability of SGC-7901cells(P<0.05),increased the protein levels ofβ-catenin,c-Myc and cyclin D1(P<0.05)and the activity of TOPflash/FOPflash dual-luciferase reporter gene(P<0.05).PRRX2interacted withβ-catenin protein in gastric cancer cells.CONCLUSION:PRRX2promotes the viability and migration ability of gastric cancer cells,which may be related to Wnt/β-catenin signaling pathway.
作者
王倩
陈冬玲
张瓅方
卞华
WANG Qian;CHEN Dong-ling;ZHANG Li-fang;BIAN Hua(Zhang Zhongjing College of Traditional Chinese Medicine,Nanyang Institute of Technology, Nanyang 473004, China;Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation, Nanyang Institute of Technology, Nanyang 473004, China)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2018年第3期410-416,共7页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81373627)
河南省张仲景方药与免疫调节重点实验室开放课题资助项目(No.kfkt201703)
