VEGF、TNF-α、miR-18a和MCM7在子宫肌瘤组织中的表达及临床意义

Expression and clinical significance of VEGF,TNF-α,miR-18a and MCM7 in uterine myoma tissues

目的分析血管内皮生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)、微小核糖核酸-18a(miR-18a)、微小染色体维持蛋白7(MCM7)在子宫肌瘤组织中的表达及临床意义。方法通过免疫组织化学法测定43例子宫肌瘤组织和正常子宫肌层组织中的VEGF、TNF-α、MCM7、孕激素受体(PR)及雌激素受体(ER)表达情况,通过原位杂交法测定miR-18a表达情况,并对结果进行比较分析。结果子宫肌瘤组织VEGF、TNF-α、MCM7、PR、ER阳性率均明显高于正常子宫肌层组织,miR-18a阳性率明显低于正常子宫肌层组织(P<0.05);Spearman相关分析结果显示,PR、ER水平与VEGF、TNF-α、MCM7均呈明显正相关,与miR-18a呈明显负相关;随着肿瘤大小增加以及肿瘤数目增多,VEGF、TNF-α、MCM7阳性率逐渐升高,而miR-18a阳性率逐渐降低。结论 VEGF、TNF-α、MCM7、miR-18a异常表达可能在子宫肌瘤发生及发展中起到了重要作用。

Objective To analyze the expression and clinical significance of VEGF,TNF-α,miR-18a and MCM7 in uterine myoma tissues.Methods The expression of VEGF,TNF-α,MCM7,PR and ER in 43 cases of uterine myoma tissues and normal uterine myometrial tissues were detected by immunohistochemistry,the expression of miR-18a was detected by situ hybridization method.The expression results were compared and analyzed.Results The positive rates of VEGF,TNF-α,MCM7,PR and ER in uterine myoma tissues were significantly higher than those in normal uterine myometrial tissues,the miR-18a positive rates was significantly lower than that in normal uterine myometrial tissues(P<0.05);the Spearman correlation analysis results showed that the levels of PR and ER had significantly positive correlation with the levels of VEGF,TNF-αand MCM7,and had significant negative correlation with the miR-18a level;the positive rates of VEGF,TNF-αand MCM7 were gradually elevated with the tumor enlargement and tumor number increase,while the miR-18a positive rate was gradually decreased.Conclusion VEGF,TNF-α,MCM7 and miR-18a abnormal expression may play an important role in the occurrence and development of uterine myoma.