HPLC法测定维吾尔药材阿勃勒中指标性成分的含量

Determination of the indicative components in Uygur medicine Abole by HPLC

目的筛选阿勃勒药材中的指标性成分,并建立含量测定方法,为阿勃勒的质量控制提供依据。方法采用HPLC法。Diamosil-C18色谱柱(250 mm×4.6 mm,5μm);乙腈-1 mL·L-1磷酸(47∶53)为流动相;检测波长为254nm;流速为1.0mL·min-1;柱温为30℃。结果大黄酸为阿勃勒中的特征性成分,大黄酸质量浓度在1.68~84.00μg·mL-1内呈良好的线性关系(r=0.999 8),平均加样回收率为99.87%,RSD值为1.01%。结论该方法操作简便、准确,所测阿勃勒中大黄酸的含量结果真实、可靠,可用于阿勃勒及其制剂的质量控制,为进一步提高阿勃勒的质量标准提供依据。

Objective To screen the indicative components,to establish an HPLC method for detecting the components from Abole,and to provide a basis for Abole quality control.Methods HPLC analysis was performed on a Diamosil-C18column(250mm×4.6mm,5μm)with the mobile phase made up of acetonitrile and1mL·L-1phosphoric acid in water(47∶53),the detection wavelength was254nm,the flow rate was1.0mL·min-1and the column temperature was30℃.Results Rhein was the characteristic component of Abole.The linear range of rhein was1.68-84.00μg·mL-1(r=0.9998),and the average recovery was99.87%(RSD=1.01%).Conclusion The method is simple and accurate for the determination of components from Abole,which is helpful to control the quality of Abole and its related preparations.