摘要
目的筛选阿勃勒药材中的指标性成分,并建立含量测定方法,为阿勃勒的质量控制提供依据。方法采用HPLC法。Diamosil-C18色谱柱(250 mm×4.6 mm,5μm);乙腈-1 mL·L-1磷酸(47∶53)为流动相;检测波长为254nm;流速为1.0mL·min-1;柱温为30℃。结果大黄酸为阿勃勒中的特征性成分,大黄酸质量浓度在1.68~84.00μg·mL-1内呈良好的线性关系(r=0.999 8),平均加样回收率为99.87%,RSD值为1.01%。结论该方法操作简便、准确,所测阿勃勒中大黄酸的含量结果真实、可靠,可用于阿勃勒及其制剂的质量控制,为进一步提高阿勃勒的质量标准提供依据。
Objective To screen the indicative components,to establish an HPLC method for detecting the components from Abole,and to provide a basis for Abole quality control.Methods HPLC analysis was performed on a Diamosil-C18column(250mm×4.6mm,5μm)with the mobile phase made up of acetonitrile and1mL·L-1phosphoric acid in water(47∶53),the detection wavelength was254nm,the flow rate was1.0mL·min-1and the column temperature was30℃.Results Rhein was the characteristic component of Abole.The linear range of rhein was1.68-84.00μg·mL-1(r=0.9998),and the average recovery was99.87%(RSD=1.01%).Conclusion The method is simple and accurate for the determination of components from Abole,which is helpful to control the quality of Abole and its related preparations.
作者
郑梦成
高艳
郭爽
孙如煜
杜守颖
白洁
陈菊
ZHENG Mengcheng;GAO Yan;GUO Shuang;SUN Ruyu;DU Shouying;BAI Jie;CHEN Ju(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;Xinjiang Yinduolan Uygur Medicine Limited Company,Urumqi 830000,China)
出处
《西北药学杂志》
CAS
2018年第2期149-152,共4页
Northwest Pharmaceutical Journal
基金
北京市优秀人才青年骨干计划项目(编号:2016000020124G076)