中华鳖GHRL基因SNPs的筛选及生长性状的关联分析

SNPS DETECTION OF GHRL GENE AND ITS ASSOCIATION WITH GROWTH TRAITS OF CHINESE SOFT-SHELL TURTLE(PELODISCUS SINENSIS)

为探究GHRL基因多态性对中华鳖(Pelodiscus sinensis)生长性状的影响,采用直接测序法在GHRL基因上检测到14个单核苷酸多态性位点C289T、G501T、T738C、G776T、A841G、T885C、T2960C、A2987T、G3390A、A3857C、G4718A、T4820C、A4850C、T4979C。随机选取同批繁殖的120只中华鳖用飞行时间质谱法进行SNPs位点的分型,并分析与生长性状的相关性。检测结果显示,所有SNP位点均符合HardyWeinberg平衡状态(P>0.05)。方差分析结果显示,C289T位点CT、CC基因型的5项生长数据均显著高于TT基因型(P<0.05)。S2位点AB基因型的体重、背甲长、背甲宽和裙边宽4项数据均显著高于AA基因型(P<0.05)。G3390A位点AG基因型的背甲长、背甲宽2项数据显著高于AA基因型(P<0.05)。G4718A位点AG基因型的背甲长、背甲宽、裙边宽3项数据显著高于AA基因型(P<0.05)。在GHRL基因上获得的SNP位点可能影响着中华鳖的生长性状或与之紧密连锁,可为中华鳖分子辅助育种提供助力与参考。

To study the association between GHRL gene and growth traits of Chinese Soft-shell Turtle(Pelodiscus sinensis),In this study,we detected and found14SNPs of GHRL gene of Pelodiscus Sinensis by DNA sequencing:C289T,G501T,T738C,G776T,A841G,T885C,T2960C,A2987T,G3390A,A3857C,G4718A,T4820C,A4850Cand T4979C.The genotype and gene frequency of these SNPs in120Chinese Soft-shell Turtle were assayed by usingthe method of Bio-Mass Spectrometry.The result showed that all the loci were found in Hardy-Weinberg equilibriumin the Chinese Soft-shell Turtle population.The growth traits(body weight,body length,body width,body height andcalipash length)of CT and CC diplotype were significantly higher than those of TT diplotype in locus C289T(P<0.05).The growth traits(Body weight,body length,body width and calipash length)of AB diplotype were significantly higherthan those of AA diplotype in locus S2(P<0.05).The growth traits(Body length and body width)of AG diplotype weresignificantly higher than those of AA diplotype in locus G3390A(P<0.05).The growth traits(Body length,body widthand calipash length)of AG diplotype were significantly higher than those of AA diplotype in locus G4718A(P<0.05).The results suggested that those loci identified in our study were closely related to the growth performance of ChineseSoft-shell Turtle;therefore it could demonstrating great potentials to be used as a potential molecular markers for better breeding.