ST195耐碳青霉烯鲍曼不动杆菌携带bla_(OXA-23-like)转座子的分析

Analysis of ST195 Acinetobacter Bauman Acinetobacter Carrying bla_(OXA-23-like) Transposon

目的分析广州市两家三甲医院ICU患者分离的ST195耐碳青霉烯鲍曼不动杆菌bla_(OXA-23-like)转座子的型别以及ST195菌株的同源性。方法选用本课题组已鉴定过的31株ST195、11株ST365、3株ST381和1株ST75耐碳青霉烯鲍曼不动杆菌,PCR法扩增携带bla_(OXA-23-like)的转座子Tn2006、Tn2007、Tn2008和Tn2009片段,PCR产物测序后经Blast比对确定转座子型别;PFGE分析31株ST195耐碳青霉烯鲍曼不动杆菌同源性。结果 31株ST195和11株ST365全部携带Tn2006,3株ST381和1株ST75检出Tn2009,未检出Tn2007和Tn2008。PFGE结果显示来自两家医院的ST195菌株可聚为一簇。结论携带Tn2006的ST195菌株可能在广州市不同医院间克隆传播,提示应加强ST195鲍曼不动杆菌监测,采取必要措施预防ST195菌株在院内和院间的传播。

Objective To identify the type of ST195resistant to carbapene-penicillium baumannii blaoxA-23-like transposon and the homology of ST195bacterial strain isolated from ICU patients of two hospitals in Guangzhou.Metheds31ST195,11ST365,3ST381,and1ST75which had been identified as carbapene-resistant acinetobacter baumannii were selected and blaOXA-23-like-carrying transposons Tn2006、Tn2007、Tn2008,and Tn2009were amplified by PCR,and the PCR products were sequenced.Sequence alignment and analysis were performed on the NCBI website using the BLAST program.31ST195strains were analyzed for homology by Pulsed field gel electrophoresis(PFGE).Results The PCR results revealed31ST195and11ST365strains all carried Tn2006,Tn2009was detected in3ST381and1ST75strains,and no strains harbored Tn2007and Tn2008.All ST195strains from two hospitals were grouped into one cluster by PFGE.Conclusion The ST195carbapenem-resistant A.baumannii strains carrying Tn2006may disseminate clonally in different hospitals in Guangzhou.Surveillance for ST195strains and prevention of dissemination of ST195strains in hospital should be strengthened.