舌鳞状细胞癌中环状RNA的表达谱特征

Expression Profile of CircularRNA in Tongue Squamous Cell Carcinoma

【目的】检测环状RNA(circRNA)在舌鳞状细胞癌组织及对应癌旁组织中表达谱的变化。【方法】利用高通量circRNA芯片检测技术,分析4对舌鳞状细胞癌组织及对应癌旁组织中circRNA表达谱的差异,确定两种组织中2倍以上变化并有显著差异(P<0.05)的circRNA为差异表达circRNA,通过将原始数据进行质控、过滤、归一化后,筛选出差异表达的circRNA,并对其进行功能分析。【结果】芯片筛选出差异表达circRNA 17 171条,其中癌灶组织相对于癌旁组织高表达的共9 982条,低表达的共7 189条。【结论】在舌鳞状细胞癌组织与对应癌旁组织中,circRNA表达谱发生了显著变化,部分差异表达的circRNA可能通过竞争性结合微小RNA调控舌鳞状细胞癌的发生发展。

【Objective】To analyze the expression profile variation of circle RNA(circRNA)in tongue squamous cell carcinoma(TSCC)tissue and para-carcinoma tissue.【Methods】CircRNA microarray technology was performed to inspect the difference of circRNA expression in4cases of TSCC tissues and4cases of para-carcinoma tissues,and then make analysis after the quality control and homogenization of raw data,to identify which have more than2times variation and significant difference(P<0.05)by statistical analysis as circRNA with differential expression.To perform functional analysis on circRNA with differential expression.【Results】Compared with para-carcinoma tissue,there were17171circRNA differentially expressed in TSCC tissue,while9982increase more than2times and7189reduce more than2times.【Conclusion】circRNA expression profile in TSCC changes significantly comparing with the para-carcinoma tissue,some differentially expressed circRNA may regulate the occurrence and progression of TSCC through a competitive combination of miRNA.