辣蓼黄酮乙酸乙酯部分对脂多糖诱导内毒素血症小鼠生化指标和炎性细胞的影响

Effects of FEA from Polygonum hydropiper L. on Biochemical Indexes and Inflammatory Cytokines in Mice with Endotoxemia Induced by Lipopolysaccharide

目的观察辣蓼黄酮乙酸乙酯(FEA)部分对内毒素血症小鼠生化指标和炎性细胞的影响,探讨其作用机制。方法采用酶解-超声偶联法提取辣蓼全草中总黄酮,结合大孔树脂纯化富集获得辣蓼黄酮乙酸乙酯(FEA)部分,脂多糖(LPS)刺激小鼠建立内毒素血症模型。实验小鼠分为空白组、模型组和FEA高、中、低剂量组,各给药组给予相应浓度药液。测定肠组织丙二醛(MDA)和髓过氧化物酶(MPO)的含量,肝组织总抗氧化能力(T-AOC)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性,血清还原型谷胱甘肽(GSH)含量与溶菌酶(LZM)、酸性磷酸酶(ACP)的活性,RT-PCR检测肺组织肿瘤坏死因子-α(TNF-α)、干扰素(IFN)-α、IFN-γ和白细胞介素-2(IL-2)含量。结果与空白组比较,模型组小鼠肠组织MDA、MPO和血清ACP水平升高,肝组织T-AOC、T-SOD、GSH-Px和血清GSH、LZM水平降低,血清、肠组织和肝组织TNF-α升高,肺组织TNF-α、IFN-α、IFN-γ和IL-2 m RNA表达升高;与模型组比较,FEA各剂量组小鼠肠组织MDA、MPO和血清ACP水平降低,FEA中、高剂量组肝组织T-AOC、T-SOD、GSH-Px和血清GSH、LZM水平升高,FEA各剂量组小鼠血清、肠组织和肝组织TNF-α含量显著降低,肺组织TNF-α、IFN-α、IFN-γ和IL-2 m RNA表达显著降低;FEA高剂量组较模型组小鼠肺组织、回肠和结肠病理形态明显改善。结论 FEA部分能降低LPS诱导小鼠内毒素血症的炎性损伤,对机体具有保护作用。

Objective To observe the effect of flavonoids ethyl acetate(FEA)from Polygonum hydropiper L.on biochemical indexes and inflammatory cytokines in mice with endotoxemia;To expore the mechanism.Methods Total flavonoids in the whole plant of Polygonum hydropiperum L.were extracted by enzymolysis-ultrasonic coupling method.The FEA part were obtained by extracting and separating,followed with macroporous resin purification and enrichment.The animal model of endotoxemia was established by stimulation of lipopolysaccharide(LPS)in mice.Experimental mice were divided into blank group,model group,FEA high-,medium-,and low-dose groups.Each administration group was given the corresponding concentration of herb liquor.The concentration of malondialdehyde(MDA)and myeloperoxidase(MPO)in intestinal tissue,total antioxidant capacity(T-AOC),superoxide dismutase(T-SOD)activity and glutathione peroxidase(GSH-Px)activity in liver tissue,glutathione(GSH),lysozyme(LZM)and acid phosphatase(ACP)in serum were determined.The contents of tumor necrosis factor-α(TNF-α),interferon(IFN)-α,IFN-γand interleukin-2(IL-2)in lung tissue were detected by RT-PCR.Results Compared with blank group,the levels of MDA,MPO in intestinal tissue and serum ACP of model mice were increased,while T-AOC,T-SOD,GSH-Px in liver tissue,serum GSH and LZM levels were decreased;TNF-αin serum,intestinal and liver tissues were increased,the expressions of TNF-α,IFN-α,IFN-γand IL-2mRNA in lung tissue were increased.Compared with the model group,the levels of MDA,MPO in intestinal tissue and serum ACP were decreased in all dose of FEA groups;The levels of T-AOC,T-SOD,GSH-Px in liver tissue,serum GSH and LZM of FEA medium and high-dose groups were increased.The content of TNF-αin mice serum,intestinal and liver tissues of all dose of FEA groups were significantly reduced,and the expressions of TNF-α,IFN-α,IFN-γand IL-2mRNA in lung tissues were significantly decreased.The pathological morphology of mice lung,ileum and colon tissues of FEA high-dose group were significantly ameliorated than model group.Conclusion FEA can attenuate inflammation injury of endotoxemia mice induced by LPS,which has protective effects for organism.