人胎盘间充质干细胞复合多孔羟基磷灰石支架的体内异位成骨效果

The ectopic osteogenesis effect of human placenta mesenchymal stem cells co-cultured with porous hydroxyapatite scaffolds in vivo

目的:探讨多孔羟基磷灰石(hydroxyapatite,HA)支架作为人工骨替代材料的异位成骨效果。方法:将人胚胎间充质干细胞与多孔HA支架混合培养7 d,取出细胞支架复合体移植入比格犬背肌内为实验组,同时植入未与细胞共培养的多孔HA支架于比格犬背肌内为对照组,在植入第12周时取出体内异位成骨组织进行成骨效果检测。结果:植入第12周时的异位成骨组织内均有新生血管形成,新生骨组织实验组明显多于对照组。结论:多孔HA支架在体内有很好的异位成骨能力,人胚胎间充质干细胞能促进新骨的形成。

Objective:To explore the ectopic osteogenesis effect of the porous hydroxyapatite(HA)scaffolds as the artificial bone substitute materials.Methods:Cultured human placenta derived mesenchymal stem cells with porous HA scaffolds for 7 d,cell-scaffold composite were taken out to implanted in the beagle’s back muscles as the experimental group,and implanted blanket porous HA scaffolds in the beagle’s back muscles as the control group,respectively remove the ectopic osteogenesis organization at 12 weeks after implantation,then detected the osteogenesis effect of the ectopic ossification organizations.Results:Implanted at 12 weeks there had new blood supply in the ectopic ossification organizations,and the experimental group new bone tissue significantly greater than the control group.Conclusions:Porous HA ceramic scaffolds have good ectopic bone formation in vivo,and human placenta mesenchymal stem cells can promote the formation of new bone.