大豆蔗糖合成酶家族成员的全基因组鉴定及表达分析

Genome-wide Identification and Expression Analysis of Sucrose Synthase Family Members in Soybean [Glycine max (L.) Merr)]

该研究基于已公布的大豆基因组序列,旨在对大豆蔗糖合成酶(SUS)家族成员进行全基因组鉴定及表达分析,以探讨大豆SUS家族基因的生物学功能,为GmSUS基因的利用及大豆高产育种奠定理论基础。结果表明:(1)在大豆基因组中共鉴定到12个蔗糖合成酶基因(GmSUS1~GmSUS12)。(2)GmSUS蛋白之间序列一致性很高,均具有植物SUS家族蛋白特有的蔗糖合成结构域和糖基转移结构域;除GmSUS5外,其他GmSUS蛋白N端均具有一个保守的丝氨酸(Ser)磷酸化位点。(3)系统进化分析显示,GmSUS蛋白主要聚为3组(SUSⅠ~SUSⅢ),且位于同1组的GmSUS基因大部分具有相似的内含子/外显子分布模式。(4)12个GmSUS基因不均匀地分布在大豆的10条染色体上,片段复制可能导致了GmSUS基因在大豆基因组中的扩增。(5)表达特性分析表明,大豆SUS家族成员具有不同的组织表达模式,GmSUS8在大豆种子中表达量很高,GmSUS1、GmSUS7和GmSUS5在大豆根瘤中表达量很高,GmSUS3、GmSUS11和GmSUS12在所有被检测的组织均具有较高的表达。

In order to understand the biological function of soybean SUS family genes,and provides basic information for breeding of high yield varieties and functional utilization of GmSUS genes in soybean,in this study,we performed the genome-wide identification and expression analysis of sucrose synthase(SUS)gene family based on published soybean genome sequence.Our results showed that:(1)a total of12putative SUS genes(GmSUS1-GmSUS12)were identified in soybean genome.(2)The sequence identity of GmSUS proteins was very high,and all of them had the sucrose synthase domain and glycosyl transferase domain.In addition to GmSUS5,other GmSUS proteins had a conserved serine phosphorylation site at their N terminal.(3)A phylogenetic analysis showed that GmSUS proteins in soybean could be classified into three groups(SUSI-SUSⅢ),and GmSUS genes in the same group exhibited similar intron/exon pattern.(4)12GmSUS genes were unevenly located on10chromosomes of soybean,and tandem duplication was the contributor to the expansion of GmSUS genes in soybean genome.(5)Expression pattern analysis indicated that GmSUS genes exhibited differential tissue expression patterns.GmSUS8was highly expressed in seed;GmSUS1,GmSUS7and GmSUS5were highly expressed in nodule;GmSUS3,GmSUS11and GmSUS12were highly expressed in all examined tissues.