基于SSR标记的彩色马铃薯亲缘关系分析及指纹图谱构建

Genetic Relationship Analysis and Fingerprint Profile Construction of Pigmented Potato Based on SSR Markers

为了探究彩色马铃薯种质资源的遗传背景,该试验共选用22对SSR标记引物,对33份彩色马铃薯品种(系)进行遗传多样性分析以及指纹图谱构建。结果表明:(1)22对引物可扩增得到95个等位位点,其中82个为多态性位点,多态性比率达到86.31%;多态信息量(PIC)从0.168 7(STM1053)到0.991 9(STI033),平均为0.8411。(2)UPGMA聚类分析表明,在相似系数0.71处,33份供试材料分为4个主要聚类群,不同聚类群之间具有较大的遗传差异。(3)利用STM0031、STM0030、STI014、STM1029、STI001共5对SSR标记引物构建了33份彩色马铃薯材料的分子标记指纹图谱。该研究结果为彩色马铃薯育种亲本组配奠定了理论基础,有助于彩色马铃薯种质资源的快速鉴定。

Pigmented potato contains massive antioxidants such as anthocyanin and flavonoid,thus they can be used as nutrient and healthy foods.To investigate the genetic background of pigmented potato and provide effective information on parental combination for potato breeding,we used22SSR markers for genetic diversity analysis and fingerprint profile of33pigmented potato varieties(lines).The results indicated that95alleles were amplified from22SSR markers,and82alleles showed polymorphism.Polymorphism information contents(PIC)of22SSR markers ranged from0.1687(STM1053)to0.9919(STI033)with an average of0.8411.UPGMA method was used for clustering analysis.With the similarity coefficient of0.71,all the pigmented potatoes were classified into four cluster groups.A significant diversity was found among four cluster groups.Five SSR markers(STM0031,STM0030,STI014,STM1029,STI001)were used to select construct the molecular fingerprint profile of33pigmented potato varieties(lines).The research results were useful to the parental combination during breeding for pigmented potato and rapid identification of pigmented potato germplasm resources.