参附注射液五种活性成分在正常和慢性心力衰竭大鼠的药动学研究

Pharmacokinetic Study on Active Ingredients of Shenfu Injection in Normal and Chronic Heart Failure Rats

比较参附注射液中五种人参皂苷活性成分在正常和慢性心力衰竭两种模型大鼠体内的药动学过程。取SD大鼠6只以盐酸阿霉素复制慢性心力衰竭模型,作为模型组;另取6只大鼠给药等体积生理盐水,作为正常组。分别对两组尾静脉注射参附注射液,制备血浆样品,UPLC-DAD法测定五种人参皂苷成分的血药浓度,Win Nonlin6.3软件计算药动学参数并进行比较。发现给药后模型组大鼠体内的人参皂苷Rg_1、Re、Rb_1、Rc和Rd的C_(max)、AUC_(last)明显升高,Cl/F明显降低,差异有统计学意义(P<0.05)。结果表明慢性心力衰竭会影响大鼠体内参附注射液的五种人参皂苷成分的药动学特征。

To compare the pharmacokinetics of active ingredients of five ginsenosides in Shenfu Injection in normal and chronic heart failure rats.Six SD rats were given doxorubicin hydrochloride to establish the model of chronic heart failure as the model group.Another six SD rats were given isometric normal saline as the control group.Shenfu Injection was given to rats of each group,respectively.After preparation of plasma samples,the plasma concentration of five kinds of ginsenosides was determined by means of UPLC-DAD method.The pharmacokinetic parameters were calculated by WinNonlin6.3 software,and then it was compared.The results showed that the C max and AUC last of ginsenoside Rg 1,Re,Rb 1,Rc and Rd were significantly higher in the model group than that in the normal group after administration.Compared with the normal group,the Cl/F was significantly decreased in the model group,and the difference was statistically significant(P<0.05).The pharmacokinetic characteristics of five ginsenosides of Shenfu Injection in rats in vivo can be affected by chronic heart failure.