siRNA干扰β-catenin基因表达情况与喉癌Hep-2细胞增殖、凋亡和侵袭的关系

The relationship between SiRNA interference-catenin gene expression and proliferation,apoptosis and invasion of Hep-2 cells in larynx cancer

目的探讨小干扰RNA(siRNA)干扰β-链蛋白(β-catenin)基因表达对喉癌Hep-2细胞增殖、凋亡和侵袭的影响。方法前瞻性选取喉癌Hep-2细胞,随机分为空白对照组、阴性对照组和干扰组,其中干扰组转染靶向β-catenin的siRNA,阴性对照组转染空白siRNA。采用荧光定量PCR和Western-blot检测β-catenin mRNA和蛋白表达,MTT检测细胞增殖,流式细胞仪检测细胞凋亡,Transell法观察细胞侵袭能力。结果干扰组β-catenin mRNA和蛋白相对表达量分别为(0.304±0.015)和(0.234±0.065),明显低于空白对照组和阴性对照组(P<0.05);干扰组培养48 h和72 h吸光值分别为(0.721±0.250)和(0.942±0.321),明显低于空白对照组和阴性对照组(P<0.05);干扰组细胞凋亡率为(19.10±1.24)%,明显高于空白对照组和阴性对照组(P<0.05);干扰组穿膜细胞数为(22.41±3.25)个,明显低于空白对照组和阴性对照组(P<0.05)。结论干扰β-catenin基因,可抑制喉癌Hep-2细胞增殖和侵袭,促进细胞凋亡。

Objective To investigate the effect of siRNA interferenceβ-catenin gene expression on the proliferation,apoptosis and invasion of Hep-2cells in larynx cancer.Methods The larynx cancer Hep-2cells were randomly divided into blank control group,negative control group and interference group.The siRNA of targetβ-catenin were transfected in the interference group,and blank siRNA in negative control group were transfected.The expression ofβ-catenin mRNA and protein were detected by fluorescent quantitative PCR and Western-blot,cell proliferation was detected by MTT,cell apoptosis was detected by flow cytometry.Transell assay was used to observe the cell invasion ability.Results Theβ-catenin mRNA and protein expression in the interference group were(0.304±0.015)and(0.234±0.065),significantly lower than those in the blank control group and negative control group(P<0.05).The absorbance of cultured48h and72h in the interference group were(0.721±0.250)and(0.942±0.321),which were significantly lower than those in the blank control group and the negative control group(P<0.05).The apoptotic rate in the interference group was(19.10±1.24)%,which was significantly higher than that in the blank control group and the negative control group(P<0.05).The number of cells through the cell membrane in the interference group was(22.41±3.25),which was significantly lower than that in the blank control group and the negative control group(P<0.05).Conclusion Interferingβ-catenin gene expression can inhibit the proliferation and invasion,and promote cell apoptosis of larynx cancer Hep-2cells.