轮状病毒感染婴幼儿与健康婴幼儿肠道菌群对比分析

A comparative study on gut flora distribution in infantile patients with Rotavirus infection and healthy infants

目的了解轮状病毒感染婴幼儿与健康婴幼儿的肠道菌群差异,探讨益生菌在轮状病毒感染时的作用及意义。方法回顾性收集15例0~3岁轮状病毒感染患儿(轮状病毒感染组)及15份健康婴幼儿(健康对照组)的粪标本,根据16Sr DNA基因序列设计双歧杆菌、乳酸杆菌、大肠杆菌、肠球菌、拟杆菌、梭菌、梭杆菌属的特异性引物,采用SYBR GreenⅠ实时荧光定量PCR法测定7种细菌的在健康组及患儿组的数量,进行比较。结果轮状病毒感染组与健康对照组比较,肠球菌、大肠杆菌、拟杆菌属数量差异无显著性(P>0.05);轮状病毒感染组双歧杆菌、乳酸杆菌、梭菌、梭杆菌属数量明显少于健康组,差异均有统计学意义(P<0.05)。结论轮状病毒感染对婴儿肠道菌群有影响,临床治疗应注意婴儿肠道菌群的调整。

Objective To understand the difference in gut flora distribution in infants with rotavirus infection and healthy infants,and to explore the role and meaning of probiotics in rotavirus infection.Methods According to the 16 SrDNA gene sequence design for bifidobacterium,lactobacillus,enterococcus,E.coli,bacteroides,fusobacterium,fusiformis specific primers,and 15 infantile cases at 0~3 years old infected with rotavirus infection were enrolled in rotavirus infection group and 15 healthy infants and young children were listed as healthy control group.Their fecal specimens were examined by using SYBR GreenⅠreal-time fluorescent quantitative PCR method for determining the number of 7 kinds of bacteria.Results There was no significant difference between the number of enterococcus,E.coli and bacillus(P>0.05)compared with those of healthy control group and rotavirus infection group The quantity of of Bifidobacterium,Lactobacillus,Fusobacterium,Fusiformis were obviously less than those of healthy controls,and the difference had statistical significance(P<0.05).Conclusion Rotavirus infection can influence the intestinal microflora in babies,and clinicians should pay more attention on the adjustment of intestinal microflora in babies.