线栓法制作大鼠局灶性缺血性脑损伤模型方法研究

Study on Method of Establishing Focal Cerebral Ischemic Brain Damage Model in Rat

目的:提供制备大鼠局灶性缺血性脑损伤模型的方法。方法:SD雄性大鼠60只,体重260~280 g,随机分为空白对照组,假手术组,缺血性脑损伤再灌注组。缺血再灌注的时间点为1d、3d、7d、14d,每组各10只。颈部正中切口,采用从颈外动脉插入线栓绕过颈总动脉分叉进入颈内动脉的方法。用0.235 mm鱼线造成大鼠中动脉血供阻断(MCAO)2 h。采用大体和组织学的观察方法,研究脑组织梗死灶及血管的变化。结果:空白组和假手术组未见梗死灶,缺血性脑损伤再灌注组有梗死灶形成。结论:该线栓法制作缺血性脑损伤模型方法稳定可靠,成功率高,死亡率低。

Objective To provide a model for the preparation of focal cerebral ischemic brain injuire in rats.Methods 60 SD male rats,weight 260~280 g,were randomly divided into blank control group,control group,the cerebral ischemic reperfusion injury group,Ischemia reperfusion time point for 1 d,3 d,7 d,14 d.Each group of 10.Neck midline incision,use the insert line from the external carotid artery bolt to bypass the method of common carotid artery into the internal carotid artery bifurcation.With a 0.235 mm line caused artery of rats in the block(MCAO)2 h.The gross and histological observation method,research on brain infarcts and the change of the blood vessels.Results Blank group and control group did not see infarcts,cerebral ischemic reperfusion injury group has formed infarcts.Conclusion Line bolt legal system for ischemic brain injury model method is stable and reliable,high success rate,low mortality rate.