电活性的石墨烯/丝素蛋白取向纳米纤维膜的制备和表征

Fabrication and characterization of electroactive aligned graphene/silk fibroin nanofibrous scaffolds

丝素蛋白和石墨烯由于具有优异性能而被广泛用于生物医学领域。为了更好的仿生天然神经的拓扑结构和电学微环境,将氧化石墨烯(GO)纳米片涂层到丝素蛋白取向纳米纤维膜上,用Vitamin C还原GO制备了具有电活性的石墨烯/丝素蛋白取向纳米纤维膜。扫描电镜(SEM)观察结果显示随着GO涂层时间的增加,纤维的直径逐渐增加,纤维的表面变得越来越粗糙并出现皱褶。电性能测试结果发现用40mmol/L Vitamin C溶液50℃还原2h,取向丝素蛋白纳米纤维膜的电导率可达到1.66×10^(-2)S/cm。傅里叶变换衰减全反射红外光谱(ATR-FT-IR)、拉曼光谱(Raman spectrum)、X射线光电子能谱(XPS)的表征结果表明GO成功地涂层到取向丝素蛋白纳米纤维膜上并且被Vitamin C有效还原。

Silk fibroin and graphene have been widely used in biomedical fields due to their excellent properties.In order to establish a better bionic natural nerve topological structure and electrical microenvironment,the electroactive graphene/silk fibroin aligned nanofibrous scaffolds were fabricated through GO nanosheets being coated onto aligned silk fibroin nanofiberous scaffolds,and then being reduced by Vitamin C.Scanning electron microscopy(SEM)showed that the diameter of the nanofibers gradually increased and the nanofibrous surface became more and more rough as well as presented corrugation with increasing the coating time of GO.The conductivity of aligned nanofibrous scaffolds was1.66×10-2S/cm after being reduced by40mmol/L Vitamin C at50℃for2h.Fourier transform attenuated total reflection infrared spectroscopy(ATR-FT-IR),Raman spectrum and X-ray photoelectron spectroscopy(XPS)displayed that GO was successfully coated onto the aligned fibroin nanofiber membrane and then reduced by Vitamin C.