摘要
以‘银叶’野鸦椿带芽茎段为材料,研究其组培快繁技术.结果表明:75%酒精处理30 s结合0.1%升汞处理8 min,消毒效果最好;MS+1.0 mg/L 6-BA+0.1 mg/L NAA为适宜的初代培养基;MS+2.0 mg/L 6-BA+0.5 mg/L NAA为适宜的增殖培养基,增殖系数达4.07;‘银叶’野鸦椿组培苗叶片的叶绿素a含量显著低于野鸦椿组培苗的叶绿素a含量.
The stem segment with bud of Euscaphis japonica‘Silver’was used to study the tissue culture of the new cultivar.The results show that the disinfection effects are the best when stem segment is disinfected in75%alcohol disinfection for30seconds andthen in0.1%HgCl2disinfection for8minuets.The suitable culture medium for primary culture is MS+1.0mg/L6-BA+0.1mg/LNAA.The suitable culture medium on subculture is MS+2.0mg/L6-BA+0.5mg/L NAA with proliferation value of4.07.And itis also showed that chlorophyll a content of Euscaphis Japonica‘Silver’seedling is significantly lower than that of Euscaphis japonica seedling.
作者
陈熙
马丽娟
蔡邦平
CHEN Xi;MA Lijuan;CAI Bangping(College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;Xiamen Botanical Garden,Xiamen, Fujian 361003, China)
出处
《宜宾学院学报》
2017年第12期116-119,共4页
Journal of Yibin University
基金
厦门市科技计划项目(3502Z20144073)
关键词
野鸦椿
新品种
组织培养
光合色素
Euscaphis japonica
a new cultivar
tissue culture
photosynthetic pigments
