三磷酸腺苷二钠氯化镁对宫内窘迫胎鼠脑保护作用的研究

Protective effect of adenosine triphosphate disodium magnesium chloride on fetal rats with intrauterine distress

目的研究三磷酸腺苷二钠氯化镁对宫内窘迫胎鼠脑组织自由基的影响和病理组织学变化,探讨三磷酸腺苷二钠氯化镁对宫内窘迫胎鼠脑组织的保护作用。方法成年雌性Sprague-Dawley大鼠15只,孕鼠随机分为Sham组、I/R组和T/M组三组,每组各5只。于妊娠第19天以3.5%水合氯醛腹腔注射麻醉,通过无损伤动脉钳钳夹双侧子宫、卵巢血管20 min建立缺血缺氧宫内窘迫模型。T/M组缺血前30 min,45 mg·kg^(-1)三磷酸腺苷二钠氯化镁腹腔注射,Sham组和I/R组于相同时间给予同等体积的0.9%氯化钠注射液。Sham组只进行开关腹手术,不钳夹子宫卵巢动脉。各组孕鼠缺氧20 min后拆除动脉夹以恢复血供,并逐层关腹。各组均于手术后24 h取标本。计算胎鼠死亡率。每组随机抽取10只胎鼠取脑组织制备标本,检测组织丙二醛水平;行HE染色高倍镜下观察胎鼠脑组织病理改变。结果胎鼠死亡率:I/R组胎鼠死亡率明显高于Sham组(P<0.05);T/M治疗组胎鼠死亡率较I/R组显著降低(P<0.05)。MDA含量:与Sham组比较,I/R组胎鼠脑组织MDA含量显著增高(P<0.05),但T/M组胎鼠脑组织MDA活性显著降低(P<0.05)。结论三磷酸腺苷二钠氯化镁能够减轻对宫内窘迫胎鼠脑组织细胞损伤,对缺血缺氧脑细胞有保护作用。

Objective To study the effect of adenosine triphosphate disodium chloride on the free radicals of brain tissue in intrauterine fetal rats and to explore the protective effect of adenosine triphosphate disodium chloride on brain tissue of intrauterine fetal rats.Methods Fifteen adult female Sprague-Dawley rats were randomly divided into sham group,I/R group and T/M group.Five was in each group.On the19th day of gestation,3.5%chloral hydrate was injected intraperitoneally with anesthesia.The model of ischemia and hypoxia and intrauterine distress was established by20hours of uterine and ovarian vascular occlusion.In the T/M group,30min and45mg·kg-1were injected intraperitoneally,and the same volume of0.9%sodium chloride was given to the Sham group and I/R group at the same time.Sham group only switched abdominal surgery,not clamped the uterine ovarian artery.Each group of pregnant rats under hypoxia states for20min after removal of the arterial clip to restore blood supply,and layer by layer off the abdomen.The rats were sacrificed at24h after operation.Calculate fetal mortality.Each group randomly selected10fetal rats to prepare brain tissue samples to detect the level of malondialdehyde in tissues.HE staining was used to observe the pathological changes of fetal brain tissue.Results The mortality of fetal rats in I/R group was significantly higher than that in Sham group(compared with Sham group,the content of MDA in brain tissue of I/R group was significantly higher than that of Sham group(P<0.05),but the activity of MDA in brain tissue of T/M group was significantly decreased(P<0.05).Conclusions Sodium adenosine triphosphate disodium chloride can reduce the damage of brain tissue cells in intrauterine fetal rats,and protect the brain cells from ischemia and hypoxia.