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毛柄短肠蕨抗氧化及体外抑制α-葡萄糖苷酶活性研究 被引量:3

Study on antioxidant and α-glucosidase inhibitory activities of Allantodia dilatata
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摘要 用乙醇回流提取法获得毛柄短肠蕨(Allantodia dilatata)的提取物,通过紫外-可见分光光度法对提取物中多酚类化合物含量进行测定,实验结果显示提取物中多酚类化合物含量为16.88%;采用DPPH法和ABTS法对提取物进行抗氧化活性测定,2种方法测得其抗氧化活性的IC_(50)值分别为28.0μg/mL和30.0μg/mL,结果表明提取物具有显著的抗氧化活性;实验测得提取物在体外抑制α-葡萄糖苷酶活性的IC_(50)值为(40.8±5.4)μg/mL,表明毛柄短肠蕨具有良好的降糖活性. In order to evaluate the antioxidant andα-glucosidase inhibitory activities of Allantodia dilatata,the total polyphenols components,as well as the clearance rate of DPPH radical and ABTS radical were detected with UV-Visible spectrophotometric methods.In addition,the inhibitory effect onα-glucosidase was also determined.The results showed that the total phenolics content of the methanol extracts was 16.88%.Further studies showed that the methanol extracts of A.dilatata had certain scavenging ability on DPPH and ABTS free radicals,the IC 50 were 28.0μg/mL and 30.0μg/mL,respectively.In addition,the extracts also exhibited theα-glucosidase in vitro with IC 50 of(40.8±5.4)g/mL and indicated that crude extracts has good hypoglycemic activity.
作者 李红锐 陆怀 黄相中 孙静贤 田凯 阮榕生 肖健 蒋孟圆 LI Hong-rui;LU Huai;HUANG Xiang-zhong;SUN Jingxian;TIAN Kai;RUAN Rong-sheng;XIAO Jian;JIANG Meng-yuan(Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission and Ministry of Education of China,Yunnan Minzu University,Kunming 650500,China)
出处 《云南民族大学学报(自然科学版)》 CAS 2018年第2期91-94,共4页 Journal of Yunnan Minzu University:Natural Sciences Edition
基金 国家自然科学基金(81460535 21262047) 云南省教育厅科学研究基金(2017YJS058)
关键词 毛柄短肠蕨 多酚含量 抗氧化性 抑制α-葡萄糖苷酶活性 Allantodia dilatata polyphenol content antioxidant α-glucosidase inhibitory activity
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