不同采样方法检测某高校公共设施表面细菌污染的对比研究

A Comparative Study of Bacterial Contamination on the Surface of Public Facilities in a University was Examined by Different Sampling Methods

目的 :比较不同采样方法在检测高校公共设施表面菌落总数的差异,从而寻找更适合于检测不同表面质地的公共设施的采样方法;方法:在某高校中随机选取教室和宿舍门把手、餐厅内餐桌椅、图书馆图书等公共设施和物品的表面,分别应用涂抹法和贴斑法采集并检测相同物体表面菌落总数,比较不同采样方法的差异性;结果:对门把手等光滑物体表面菌落总数检测分析,得出两种采样方法差异不显著(P>0.05);而对图书馆图书等非光滑物体表面的检测,得出两种采样方法差异显著(P<0.05),贴斑法的检测结果明显高于涂抹法;结论不同表面质地的物体表面需要用不同的采样方法,其中,非光滑物体表面需要采用贴斑法进行采样,才能够保证检测结果的准确性。

Objective to compare the differences in the total number of bacterial colonies on the surface of public facilities in colleges and universities in order to find out the sampling method of public facilities more suitable for testing different surface textures;Method randomly selected in a university classroom and dormitory door handles,the eat desk and chair inside dining-room,library books and other public facilities and the surface of objects,respectively applied daub and post spot method to collect and detect the same surface total number of colonies,comparing the differences of different sampling methods;Results there was no significant difference between the two sampling methods(P BBB 0.05).On the surface of non-smooth surfaces such as library books,the difference between the two sampling methods is significant(P<0.05),and the detection result of the patch method is significantly higher than that of the daub method.Conclusion the surface of different surface texture needs different sampling methods,among which,the surface of non-smooth surface needs to be sampled by spot method,so as to ensure the accuracy of test results.