凤仙花的指纹图谱研究及2种1,4-萘醌成分的含量测定

Establishment of fingerprints and simultaneous determination 2 kinds of 1,4-naphthoquinone in Impatiens balsamina L.

目的建立凤仙花的HPLC指纹图谱,为凤仙花的质量控制提供可靠方法;对不同产地凤仙花中的指甲花醌(HNQ)和指甲花甲醚(MeONQ)进行含量测定。方法采用Agilent TC-C18(2)色谱柱(250mm×4.6mm,5μm);乙腈(A)-10mL·L^(-1)乙酸(B)为流动相;流速为1.0mL·min^(-1);梯度洗脱;柱温:30℃;检测波长:280nm。结果方法学验证结果显示,指甲花醌和指甲花甲醚质量浓度分别在0.004~0.400(r=0.999 3)和0.008~0.800mg·mL^(-1)(r=0.999 6)范围内线性关系良好;其平均加样回收率分别为98.56%和102.75%;RSD值分别为1.92%和1.96%(n=6)。建立了凤仙花HPLC的指纹图谱共有模式,标定10个共有峰,10批样品指纹图谱相似度在0.837~0.957范围内。将指甲花醌和指甲花甲醚作为主要特征成分进行含量测定,结果显示,不同产地10批凤仙花中指甲花醌和指甲花甲醚的含量范围分别为0.011 3~0.738 6和1.784 9~5.881 3mg·g-1,其中以北京市自种凤仙花的指甲花醌和指甲花甲醚含量最高。结论该测定方法简便、准确、重复性好。不同产地凤仙花的指纹图谱相似度和共有峰相对峰面积差异较大,主要特征成分含量也相差悬殊,说明药材质量优劣与产地有很大关系。

To establish the HPLC fingerprints of chemical constituents in Impatiens balsamina L.and provide the basis for the quality evaluation.To simultaneously determine the contents of lawsone(H N Q)and 2-m ethoxy-l,4-naphthoquinone(M e-ONQ)from different habitats.The ethanol extracts of samples were analyzed on an Agilent TC-C18(2)column(250 mm X 4.6 m m,5 jum)eluted with the mobile phase of acetonitrile(A)and 10 mL.L 1 acetic acid/water solution(B).The flow rate of the mobile phase,the column temperature,and the detection wavelength were set at 1.0 mL.min 1,30°C,and 280 nm,respectively.For the method validation,HNQ and MeONQ in Impatiens balsamina L.showed a good linearity in the range of 0.004-0.400(r=0.999 3)and 0.008-0.800 mg.mL 1(r=0.999 6),and the mean recoveries were 98.56%and 102.75%with the RSD(n=6)of 1.92%and 1.96%respectively.HPLC fingerprint of Impatiens balsamina L.was established,10 main marker peaks were selected.The fingerprint similarities of the 10 sample batches were distributed between 0.837-0.957.The quantitative results showed that the contents of H NQ and MeONQ were in the range of 0.Oil 3-0.738 6 and 1.784 9-5.881 3 mg.g 1,respectively.Meanwhile,the contents of 2 kinds of 1,4-naphthoquinone in Impatiens balsamina L.cultivated in Beijing were higher than those cultivated in other districts.This method was proved to be simple,accurate and repeatable.There were obvious differences of fingerprint similarities or the contents of HNQ and MeONQ from different areas,which revealed the quality of this plant is closely linked to its growing areas.